夏威夷半边莲属植物微卫星位点的特征及其在其他属中的可转移性
Characterization of microsatellite loci in and transferability to other genera in the Hawai'ian lobelioid group.
作者信息
Fant Jeremie B, Fluckes Mereida, James Evana, Noble Hilary, Wood Jordan
机构信息
Plant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USA.
Plant Biology and Conservation Northwestern University O. T. Hogan Hall, 2205 Tech Drive Evanston Illinois 60208 USA.
出版信息
Appl Plant Sci. 2019 Nov 11;7(11):e11303. doi: 10.1002/aps3.11303. eCollection 2019 Nov.
PREMISE
Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of (Campanulaceae).
METHODS AND RESULTS
Potential microsatellite markers were identified from two sources; 28 were developed for and an additional 12 markers from a previously published study of . Primer pairs were tested on 30 individuals of and 24 individuals of to provide measures of genetic diversity and inbreeding. We assessed cross-species amplification in an additional 13 taxa that represented all six genera within the Hawai'ian lobelioid group to determine the broader applicability of the markers.
CONCLUSIONS
Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai'ian lobelioid group.
前提
开发微卫星标记以测量风铃草科（Campanulaceae）迁地保护收集品的遗传多样性和相关性。
方法与结果
从两个来源鉴定出潜在的微卫星标记；为风铃草科开发了28个标记，并从先前发表的一项关于风铃草科的研究中另外获得12个标记。在30个风铃草属个体和24个半边莲属个体上测试引物对，以提供遗传多样性和近亲繁殖的测量数据。我们在另外13个分类群中评估了跨物种扩增，这些分类群代表了夏威夷半边莲类群中的所有六个属，以确定这些标记的更广泛适用性。
结论
结果表明，这些引物将为两种风铃草科物种的迁地保护收集品的遗传多样性和相关性提供有用的估计。此外，我们还证明了这些标记在夏威夷半边莲类群中几个物种的群体遗传学研究中的广泛适用性。
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