Programa de Pós-graduação em Biotecnologia aplicada à Agropecuária, Universidade Federal Rural da Amazônia, Belém, Pará, 66077-530, Brazil.
Programa de Pós-graduação em Agronomia, Universidade Federal Rural da Amazônia, Belém, Pará, 66077-530, Brazil.
Mol Biol Rep. 2020 Apr;47(4):2985-2989. doi: 10.1007/s11033-020-05301-0. Epub 2020 Feb 7.
Platonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (H) varied from 0.74 (PI27) to 0.12 (PI31) and observed heterozygosity (H) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations.
星苹果是一种原产于巴西的异花授粉和无性繁殖的果树。果实的生产主要是通过开发自然种群获得的，因此可以评估遗传结构对植物生产的影响。为此，共显性和多等位基因标记，如微卫星，是最合适的，但需要为该物种开发这些标记。因此，本工作的目的是为星苹果开发和验证微卫星标记。我们使用罗氏 454 GS FLX 测序平台对单一星苹果基因型进行测序，共鉴定到 1702 个微卫星序列。基于一些前提条件，我们可以开发 50 对引物进行测试。22 对引物成功扩增了片段，并在来自巴西帕拉州东北部种质库的 31 个星苹果基因型中进行了测试。其中 13 对引物具有多态性，每个位点的等位基因数从 5 个（PI18 和 PI27）到 2 个（PI08、PI25、PI31、PI33 和 PI37）不等。期望杂合度（H）从 0.74（PI27）到 0.12（PI31）不等，观测杂合度（H）从 1.00（PI25）到 0.00（PI08、PI31、PI33 和 PI37）不等。主坐标可以将星苹果的基因型聚类分离，我们可以得出结论，这些引物可以估计星苹果种群的遗传多样性。
