Xiao Xianghui, Lu Quanwei, Liu Ruixian, Gong Juwu, Gong Wankui, Liu Aiying, Ge Qun, Li Junwen, Shang Haihong, Li Pengtao, Deng Xiaoying, Li Shaoqi, Zhang Qi, Niu Doudou, Chen Quanjia, Shi Yuzhen, Zhang Hua, Yuan Youlu
1College of Agronomy, Xinjiang Agricultural University, Urumqi, China.
2State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
3 Biotech. 2019 Dec;9(12):453. doi: 10.1007/s13205-019-1984-1. Epub 2019 Nov 16.
Uridine diphosphate (UDP)-glycosyltransferases (UGTs) involved in many metabolic processes are ubiquitous in plants, animals, microorganisms and other organisms and are essential for their growth and development. Upland cotton contains a large number of UGT genes. In this study, we aimed to identify UGT family members in the genome of upland cotton ( L.) and analyze their expression patterns. Bioinformatics methods were used to identify UGT genes from the whole genome of upland cotton ( L. acc. TM-1). Phylogenetic analysis was conducted based on alignment of UGT proteins from upland cotton, and the gene structure, motif and chromosome localization were analyzed for the H subgroup of the UGT family. And the physical and chemical properties and expressions of the genes in the H subgroup of this family were also analyzed. A total of 274 UGT genes were identified from the whole genome of upland cotton and were divided into nine subgroups based on phylogenetic analyses. In subgroup H, 36 genes were distributed on 18 chromosomes. The subfamily genes were simple in the structure, 19 of its members contained two introns, and the others contained only one intron. The qRT-PCR results and transcriptomic data indicated that most of the genes had a wide range of tissue expression characteristics. And the phylogenetic analysis results and expression profiles of these genes revealed tissues and different UGT genes from this crop. Taking RNA-seq, RT-qPCR, and quantitative trait locus (QTL) mapping together, our results suggested that GhUGT6 and GhUGT105 in subgroup H of the GhUGT gene family could be potential candidate genes for cotton yield, and GhUGT16, GhUGT103 might play a vital role in fiber development.
参与许多代谢过程的尿苷二磷酸（UDP）-糖基转移酶（UGT）在植物、动物、微生物和其他生物体中普遍存在，对它们的生长发育至关重要。陆地棉含有大量UGT基因。在本研究中，我们旨在鉴定陆地棉（Gossypium hirsutum L.）基因组中的UGT家族成员并分析其表达模式。利用生物信息学方法从陆地棉（Gossypium hirsutum L. acc. TM-1）全基因组中鉴定UGT基因。基于陆地棉UGT蛋白的比对进行系统发育分析，并对UGT家族H亚组的基因结构、基序和染色体定位进行分析。同时还分析了该家族H亚组基因的理化性质和表达情况。从陆地棉全基因组中总共鉴定出274个UGT基因，并根据系统发育分析将其分为9个亚组。在H亚组中，36个基因分布在18条染色体上。该亚家族基因结构简单，其成员中有19个含有两个内含子，其他成员仅含有一个内含子。qRT-PCR结果和转录组数据表明，大多数基因具有广泛的组织表达特征。这些基因的系统发育分析结果和表达谱揭示了该作物不同的组织和UGT基因。综合RNA-seq、RT-qPCR和数量性状位点（QTL）定位分析，我们的结果表明，GhUGT基因家族H亚组中的GhUGT6和GhUGT105可能是棉花产量的潜在候选基因，而GhUGT16、GhUGT103可能在纤维发育中起重要作用。
