基因多态性与伊朗人群的膀胱癌相关。
Gene Polymorphisms Are Associated with Urinary Bladder Cancer in an Iranian Population.
作者信息
Shafieian Zahra, Bahari Gholamreza, Hashemi Mohammad, Nakhaee Alireza
机构信息
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
出版信息
Rep Biochem Mol Biol. 2019 Jul;8(2):194-199.
BACKGROUND
The present study was undertaken to evaluate the possible association between silent information regulator of transcription 1 gene () polymorphisms and risk of urinary bladder cancer (UBC) in an Iranian population.
METHODS
The polymorphisms rs3758391 T/C and rs369274325 G/A were evaluated in 120 Iranian bladder cancer patients and 118 healthy individuals as the control group. The rs369274325 G/A and rs3758391 T/C polymorphisms were genotyped using tetra-primer ARMS PCR and PCR-RFLP methods, respectively.
RESULTS
The rs3758391 TT genotype occurred significantly more frequently in the UBC patients than in the controls (13.3 vs. 1.7%) in both the additive and recessive models due to a significant difference in either of additive (TT vs. CC; OR= 9.529, P = 0.003) or recessive models (TT vs. CC + CT genotype; OR= 8.923, P = 0.002). Also, for rs369274325, the AG genotype was found in a significantly greater percentage of UBC patients than in controls (75.8 vs. 43.2%, respectively, P < 0.0001.
CONCLUSION
Our preliminary study suggests that rs3758391 T/C and rs369274325 G/A polymorphisms may confer an increased risk of bladder cancer in our patients.
背景
本研究旨在评估伊朗人群中转录沉默信息调节因子1基因（）多态性与膀胱癌（UBC）风险之间的可能关联。
方法
对120例伊朗膀胱癌患者和118名健康个体作为对照组，评估rs3758391 T/C和rs369274325 G/A的多态性。分别采用四引物扩增不应变系统PCR和聚合酶链反应-限制性片段长度多态性方法对rs369274325 G/A和rs3758391 T/C多态性进行基因分型。
结果
在加性和隐性模型中，由于加性（TT与CC；OR=9.529，P=0.003）或隐性模型（TT与CC+CT基因型；OR=8.923，P=0.002）中存在显著差异，UBC患者中rs3758391 TT基因型的出现频率显著高于对照组（分别为13.3%和1.7%）。此外，对于rs369274325，UBC患者中AG基因型的比例显著高于对照组（分别为75.8%和43.2%，P<0.0001）。
结论
我们的初步研究表明，rs3758391 T/C和rs369274325 G/A多态性可能会增加我们患者患膀胱癌的风险。
Zotero 插件