Yaghoobi Hajar, Azizi Hakim, Banitalebi-Dehkordi Mehdi, Mohammad Rezaei Fatemeh, Arsang-Jnag Shahram, Taheri Mohammad, Ghafouri-Fard Soudeh
University of Medical Sciences, Shahrekord, Iran.
Department of Medical Biotechnology, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Rep Biochem Mol Biol. 2019 Jul;8(2):200-207.
The enzyme beta-secretase 1 (BACE1) and its antisense transcript () have been implicated in the pathogenesis of Alzheimer's disease. Moreover, several lines of evidence point to their contribution in tumorigenesis.
In the present study, we evaluated expression of BACE1 mRNA () and in 54 breast cancer tissues and 54 adjacent non-cancerous tissues (ANCTs) from the same patients using quantitative real-time PCR.
was significantly down-regulated in tumoral tissues compared with ANCTs, while expression was not significantly different between tumoral tissues and ANCTs. The Bayesian Multilevel model showed a significant difference in BACE1 expression between stage 1 and 2 cancers after age-effect adjustments. expression was significantly greater in ER-positive than in ER-negative samples (P=0.01). and expression were not correlated with patient ages in any sample sets.
Significant correlations were detected between expression of these genes in both tumoral tissues and ANCTs. The current study provides evidence for differential expression in breast tissues and suggests further assessment of the role of in the pathogenesis of cancer.
β-分泌酶1（BACE1）及其反义转录本（）与阿尔茨海默病的发病机制有关。此外，有几条证据表明它们在肿瘤发生中起作用。
在本研究中，我们使用定量实时PCR评估了来自同一患者的54例乳腺癌组织和54例相邻非癌组织（ANCTs）中BACE1 mRNA（）和的表达。
与ANCTs相比，肿瘤组织中的明显下调，而肿瘤组织和ANCTs之间的表达没有显著差异。贝叶斯多级模型显示，在年龄效应调整后，1期和2期癌症之间的BACE1表达存在显著差异。ER阳性样本中的表达明显高于ER阴性样本（P = 0.01）。在任何样本集中，和的表达与患者年龄均无相关性。
在肿瘤组织和ANCTs中均检测到这些基因表达之间存在显著相关性。本研究为乳腺组织中的差异表达提供了证据，并建议进一步评估在癌症发病机制中的作用。
