Soleimani Shiva, Nasim Negin, Esfandi Farbod, Karimipoor Morteza, Kholghi-Oskooei Vahid, Naby Gol Maryam, Taheri Mohammad, Ghafouri-Fard Soudeh
GenIran Lab, Tashkhis Gene Pajohesh, Tehran, Iran.
Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2020 Winter;13(1):8-13.
The current study aimed to identify the expression levels of (), , () in gastric cancer tissues and their corresponding adjacent non-cancerous tissues (ANCTs).
SET has been first identified as a component of a fusion protein produced by chromosomal rearrangement in a patient with acute undifferentiated leukemia. Subsequently, multiple functions have been attributed to this gene in different disorders such as cancer and Alzheimer's disease. The expression of SET is regulated by ZFX, a transcription factor which has a potential role in gastric cancer.
In this case-control study, we evaluated the expression of and in gastric cancer tissues (n=28) and their corresponding ANCTs (n=28) via quantitative real-time PCR.
gene was down-regulated in tumoral tissues compared with ANCTs (expression ratio=0.25, P=0.015). However, the expression of was similar between tumoral tissues and ANCTs (expression ratio=0.97, P=0.945). We detected a significant association between the site of primary tumor and relative expression in tumoral tissues versus ANCTs, where this gene was down-regulated in all tumors originating from cardia. Based on the area under the receiver operating characteristic curve, the diagnostic power of transcription levels of in gastric cancer was 0.68. Finally, we observed remarkable correlations between expression levels of and both in tumoral tissues (R=0.38, P<0.05) and in ANCTs (R=0.23, P<0.05).
Overall, our results imply the role of SET1 in gastric cancer and potentially functional associations between this gene and ZFX in gastric tissues.
本研究旨在确定胃癌组织及其相应的癌旁非癌组织（ANCTs）中（）、、（）的表达水平。
SET最初被鉴定为一名急性未分化白血病患者染色体重排产生的融合蛋白的一个组成部分。随后，该基因在癌症和阿尔茨海默病等不同疾病中具有多种功能。SET的表达受ZFX调控，ZFX是一种在胃癌中具有潜在作用的转录因子。
在这项病例对照研究中，我们通过定量实时PCR评估了胃癌组织（n = 28）及其相应的ANCTs（n = 28）中及的表达。
与ANCTs相比，肿瘤组织中的基因表达下调（表达比 = 0.25，P = 0.015）。然而，肿瘤组织和ANCTs之间的表达相似（表达比 = 0.97，P = 0.945）。我们检测到原发肿瘤部位与肿瘤组织相对于ANCTs中的相对表达之间存在显著关联，其中该基因在所有源于贲门的肿瘤中均下调。基于受试者工作特征曲线下面积，胃癌中转录水平的诊断能力为0.68。最后，我们观察到肿瘤组织（R = 0.38，P < 0.05）和ANCTs（R = 0.23，P < 0.05）中及的表达水平之间均存在显著相关性。
总体而言，我们的结果表明SET1在胃癌中的作用以及该基因与胃组织中ZFX之间潜在的功能关联。
