蓝鳍金枪鱼（Thunnus thynnus）肌红蛋白的氨基酸组成和理化性质

Amino acid composition and physico-chemical properties of bluefin tuna (Thunnus thynnus) myoglobin.

Balestrieri C, Colonna G, Giovane A, Irace G, Servillo L, Tota B

Institute of Biological Chemistry, 1st Faculty of Medicine, University of Naples, Italy.

Comp Biochem Physiol B. 1978;60(2):195-9. doi: 10.1016/0305-0491(78)90130-x.

Abstract

The heart ventricle myoglobin of Atlantic bluefin tuna has been purified and its amino acid composition has been determined. 2. The perturbing effect of guanidine hydrochloride on the molecular structure of tuna ferrimyoglobin and its corresponding apoprotein has been investigated by Soret absorbance and ultraviolet fluorescence. 3. The conformation-free energy of unfolding delta G0 has been calculated by thermodynamic treatments of the data concerning guanidine unfolding. 4. The results have been compared with other known myoglobins, particularly those of yellowfin tuna.

摘要

大西洋蓝鳍金枪鱼的心室肌红蛋白已被纯化，其氨基酸组成也已确定。2. 通过索雷特吸收光谱和紫外荧光研究了盐酸胍对金枪鱼高铁肌红蛋白及其相应脱辅基蛋白分子结构的扰动作用。3. 通过对胍变性数据进行热力学处理，计算出了去折叠的构象自由能ΔG0。4. 已将结果与其他已知的肌红蛋白进行了比较，特别是黄鳍金枪鱼的肌红蛋白。