Godfraind T
Laboratory of General Pharmacodynamics and Pharmacology, Catholic University of Louvain, Brussels, Belgium.
Int Angiol. 1988 Apr-Jun;7(2 Suppl):17-9.
S 5682 is constituted by a flavonoid mixture of 90% diosmin and 10% hesperidin. Its action has been studied in vivo on microcirculation by measuring experimental alterations of capillary permeability and of venous pressure. Rats were pretreated by IV injection of 25 mg S 5682/kg, one hour before being submitted to a transitory compression of the posterior paw, resulting in reversible oedema that was estimated by plethysmography. The swelling of the paw in pretreated rats was lower than in controls (p less than 0.02), indicating a smaller increase of capillary permeability in rats treated with S 5682. A direct action on capillary permeability has been examined by measuring accumulation of IV injected Evans blue at the site of injection of zymosan, this accumulation was lower in pretreated rats than in controls (p less than 0.05). Similarly, in CFY rats, the pressure required to evoke capillary fragilisation was higher in S 5682 pretreated rats. Evans blue extravasation was also studied in the rabbit in which skin was irritated by topical application of chloroform of by gamma rays. Subcutaneous accumulation of Evans blue was lower in animals pretreated either IV or by oral route than in controls (p less than 0.05). S 5682 has a complex effect on microcirculation as indicated by a smaller increase in femoral venous pressure after ligation of homolateral iliac vein. The above experimental results indicate that S 5682 is acting at the venous side of the microcirculation.
S 5682由90%的地奥司明和10%的橙皮苷组成的类黄酮混合物构成。通过测量毛细血管通透性和静脉压的实验性改变，对其在体内对微循环的作用进行了研究。在对大鼠后爪进行短暂压迫导致可逆性水肿之前一小时，通过静脉注射25 mg S 5682/kg对大鼠进行预处理，该水肿通过体积描记法进行评估。预处理大鼠的爪肿胀程度低于对照组（p<0.02），表明用S 5682处理的大鼠毛细血管通透性增加较小。通过测量静脉注射伊文思蓝在酵母聚糖注射部位的蓄积量，研究了其对毛细血管通透性的直接作用，预处理大鼠的这种蓄积量低于对照组（p<0.05）。同样，在CFY大鼠中，诱发毛细血管脆性增加所需的压力在S 5682预处理大鼠中更高。还在兔中研究了伊文思蓝外渗情况，兔的皮肤通过局部应用氯仿或γ射线进行刺激。静脉注射或口服预处理动物的伊文思蓝皮下蓄积量低于对照组（p<0.05）。如在结扎同侧髂静脉后股静脉压升高较小所示，S 5682对微循环有复杂作用。上述实验结果表明，S 5682作用于微循环的静脉侧。
