Molecular Entomology Laboratory, Deparment of Plant Protection, Faculty of Agriculture, Ankara University, Ankara 06100, Turkey.
Molecular Entomology Laboratory, Deparment of Plant Protection, Faculty of Agriculture, Ankara University, Ankara 06100, Turkey.
Acta Trop. 2020 Mar;203:105294. doi: 10.1016/j.actatropica.2019.105294. Epub 2019 Dec 10.
Chemical insecticides are the main tool for vector control worldwide with pyrethroids and the larvicide diflubenzuron (DFB) used extensively against the primary West Nile virus vector Culex pipiens. In this study we monitored the presence, frequency and geographical distribution of the Chitin synthase 1 gene mutations I1043L, I1043M associated with striking diflubenzuron resistance and the knock down resistance mutation L1014F associated with pyrehtroid resistance, in Cx. pipiens populations from Turkey. DFB resistance mutations I1043L, I1043M were found at high frequencies ranging from 15.7% to 37.5% and 25% to 52.7% in all study sites (first report for the presence of DFB resistant mutations, anywhere outside Italy). The L1014F pyrethroid resistance mutation was also present at relatively high frequencies, ranging from 40% to 50%. The high frequency of both DFB and pyrethroid resistance mutations are possibly associated with the intense use of these insecticides in agricultural and mosquito control applications in the region. Given the limited number of available insecticides for vector control these findings are of major importance for public health in Turkey and neighboring countries.
化学杀虫剂是全球病媒控制的主要工具，拟除虫菊酯和杀幼虫剂除虫脲（DFB）被广泛用于对抗主要的西尼罗河病毒载体库蚊。在这项研究中，我们监测了土耳其库蚊种群中与除虫脲抗性相关的几丁质合成酶 1 基因突变 I1043L、I1043M 的存在、频率和地理分布，以及与拟除虫菊酯抗性相关的击倒抗性突变 L1014F。在所有研究地点，DFB 抗性突变 I1043L、I1043M 的频率范围为 15.7%至 37.5%和 25%至 52.7%（首次在意大利以外的任何地方报告 DFB 抗性突变的存在）。L1014F 拟除虫菊酯抗性突变的频率也相对较高，范围为 40%至 50%。DFB 和拟除虫菊酯抗性突变的高频率可能与该地区农业和蚊虫控制应用中这些杀虫剂的大量使用有关。鉴于可用于病媒控制的杀虫剂数量有限，这些发现对土耳其和邻国的公共卫生具有重要意义。
