Lucchesi Valentina, Grimaldi Lorenzo, Mastrantonio Valentina, Porretta Daniele, Di Bella Letizia, Ruspandini Tania, Di Salvo Martino Luigi, Vontas John, Bellini Romeo, Negri Agata, Epis Sara, Caccia Silvia, Bandi Claudio, Urbanelli Sandra
Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.
Department of Earth Sciences, Sapienza University of Rome, 00185 Rome, Italy.
Insects. 2022 Nov 30;13(12):1109. doi: 10.3390/insects13121109.
Insecticide resistance is a major threat challenging the control of harmful insect species. The study of resistant phenotypes is, therefore, pivotal to understand molecular mechanisms underpinning insecticide resistance and plan effective control and resistance management strategies. Here, we further analysed the diflubenzuron (DFB)-resistant phenotype due to the point-mutation I1043M in the chitin-synthase 1 gene (1) in the mosquito . By comparing susceptible and resistant strains of through DFB bioassays, molecular analyses and scanning electron microscopy, we showed that the I1043M-resistant mosquitoes have: (i) a striking level of DFB resistance (i.e., resistance ratio: 9006); (ii) a constitutive 11-fold over-expression of the 1 gene; (iii) enhanced cuticle thickness and cuticular chitin content. is one of the most important vector species in Europe and the rapid spread of DFB resistance can threaten its control. Our results, by adding new data about the DFB-resistant phenotype, provide important information for the control and management of insecticide resistance.
杀虫剂抗性是挑战有害昆虫物种控制的一个主要威胁。因此，对抗性表型的研究对于理解支撑杀虫剂抗性的分子机制以及制定有效的控制和抗性管理策略至关重要。在此，我们进一步分析了由于蚊子几丁质合酶1基因（1）中的点突变I1043M导致的敌氟隆（DFB）抗性表型。通过DFB生物测定、分子分析和扫描电子显微镜比较易感和抗性品系，我们发现具有I1043M抗性的蚊子有：（i）显著水平的DFB抗性（即抗性比率：9006）；（ii）1基因组成型过表达11倍；（iii）表皮厚度和表皮几丁质含量增加。 是欧洲最重要的病媒物种之一，DFB抗性的迅速传播会威胁到对它的控制。我们的结果通过添加有关DFB抗性表型的新数据，为杀虫剂抗性的控制和管理提供了重要信息。
