Department of Stomatology, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing 400037, PR China.
Department of Oral and Maxillofacial Surgery, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing 400037, PR China.
Arch Oral Biol. 2020 Feb;110:104632. doi: 10.1016/j.archoralbio.2019.104632. Epub 2019 Dec 5.
Porphyromonas gingivalis (P. gingivalis)-derived LPS is a major mediator of inflammation and can promote the resorption of alveolar bone in chronic periodontitis. Although the effect of P. gingivalis LPS on human periodontal ligament fibroblasts (hPDLFs) was already investigated by numerous studies, the change of whole transcriptional profile remains undefined. The aim of this study was to investigate P. gingivalis LPS induced whole transcriptional profile in hPDLFs and the expression of the genes associated with the periodontitis.
RNA-seq was performed on hPDLFs treated with or without P. gingivalis LPS. Moreover, the expression of selected inflammatory cytokines, chemokines and matrix metalloproteinases (MMPs), which contribute to periodontitis, were evaluated by quantitative RT-PCR and further measured by ELISA.
We found that an average of 12,752 genes were detected among the different groups, and 1374 differentially expressed genes (DEGs) were identified between groups with or without P. gingivalis LPS stimulation for 24 h. However, only 36 DEGs were examined in hPDLFs exposed to P. gingivalis LPS for 24 h or 72 h. Furthermore, the mRNA levels and concentrations of interleukin 8 (IL-8), IL-6, monocyte chemotactic protein 1 (MCP-1), chemokine (CXC motif) ligand 5 (CXCL5), MMP1 and MMP3 were significantly higher in hPDLFs exposed to P. gingivalis LPS for 24 h compared to the untreated hPDLFs.
The entire transcriptional profile of P. gingivalis LPS stimulation of hPDLFs was presented for the first time, which could provide an important basis and experimental direction for further research into the mechanisms of periodontitis.
牙龈卟啉单胞菌（P. gingivalis）衍生的脂多糖（LPS）是炎症的主要介质，可促进慢性牙周炎中牙槽骨的吸收。尽管许多研究已经研究了 P. gingivalis LPS 对人牙周韧带成纤维细胞（hPDLFs）的影响，但整个转录谱的变化仍未确定。本研究旨在研究 P. gingivalis LPS 诱导的 hPDLFs 全转录谱以及与牙周炎相关的基因表达。
对用或不用 P. gingivalis LPS 处理的 hPDLFs 进行 RNA-seq。此外，通过定量 RT-PCR 评估有助于牙周炎的选定炎症细胞因子、趋化因子和基质金属蛋白酶（MMPs）的表达，并通过 ELISA 进一步测量。
我们发现不同组之间平均检测到 12752 个基因，并用或不用 P. gingivalis LPS 刺激 24 小时的组之间鉴定出 1374 个差异表达基因（DEG）。然而，仅在暴露于 P. gingivalis LPS 24 小时或 72 小时的 hPDLFs 中检查了 36 个 DEG。此外，在暴露于 P. gingivalis LPS 24 小时的 hPDLFs 中，白细胞介素 8（IL-8）、IL-6、单核细胞趋化蛋白 1（MCP-1）、趋化因子（CXC 基序）配体 5（CXCL5）、基质金属蛋白酶 1 和基质金属蛋白酶 3 的 mRNA 水平和浓度显着高于未处理的 hPDLFs。
首次呈现了 P. gingivalis LPS 刺激 hPDLFs 的整个转录谱，为进一步研究牙周炎的机制提供了重要的基础和实验方向。
