miR-731 通过靶向 CiGadd45aa 对草鱼的炎症和凋亡的影响。
Effects of microRNA-731 on inflammation and apoptosis by targeting CiGadd45aa in grass carp.
机构信息
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
出版信息
Fish Shellfish Immunol. 2020 Feb;97:493-499. doi: 10.1016/j.fsi.2019.12.029. Epub 2019 Dec 12.
Ctenopharyngodon idellagrowth arrest and DNA damage-inducible 45aa (CiGadd45aa) is a member of the Gadd45 family of immune-related proteins in grass carp. There is increasing evidence that microRNAs (miRNAs) are involved in the regulation of inflammatory responses and apoptosis. However, little is known about the regulatory effects of miRNAs on CiGadd45aa expression in grass carp. In the present study, CiGadd45aa was identified as a target gene of miR-731 based on miRNA expression profiling and dual-luciferase reporter assay. Our study revealed that miR-731 targets CiGadd45aa and regulates the expression of proinflammatory factors, thereby regulating immune response in grass carp. In addition, CiGadd45aa and miR-731 were both found induced apoptosis. Hence, our findings provide a theoretical basis for exploring the molecular mechanism by which miR-731 regulates inflammation and apoptosis in grass carp.
草鱼生长停滞和 DNA 损伤诱导蛋白 45aa（CiGadd45aa）是草鱼免疫相关蛋白 Gadd45 家族的一员。越来越多的证据表明，microRNAs（miRNAs）参与了炎症反应和细胞凋亡的调节。然而，miRNAs 对草鱼 CiGadd45aa 表达的调控作用知之甚少。在本研究中，基于 miRNA 表达谱和双荧光素酶报告基因检测，鉴定出 CiGadd45aa 是 miR-731 的靶基因。我们的研究表明，miR-731 靶向 CiGadd45aa 并调节促炎因子的表达，从而调节草鱼的免疫反应。此外，还发现 CiGadd45aa 和 miR-731 都能诱导细胞凋亡。因此，我们的研究结果为探索 miR-731 调节草鱼炎症和凋亡的分子机制提供了理论依据。
Zotero 插件