生长停滞与DNA损伤诱导蛋白45-β在嗜水气单胞菌感染后激活草鱼（Ctenopharyngodon idella）的促炎细胞因子并促进吞噬作用。
Growth arrest and DNA damage inducible 45-beta activates pro-inflammatory cytokines and phagocytosis in the grass carp (Ctenopharyngodon idella) after Aeromonas hydrophila infection.
作者信息
Bai Yulin, Shen Yubang, Xu Xiao-Yan, Bai Yuqi, Fang Yuan, Zhang Meng, Miao Yiheng, Zhang Xueshu, Li Jia-le
机构信息
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Chinese Academy of Fishery Sciences, Wuxi, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
出版信息
Dev Comp Immunol. 2018 Oct;87:176-181. doi: 10.1016/j.dci.2018.06.010. Epub 2018 Jun 19.
Growth arrest and DNA damage inducible 45-beta (Gadd45B) is essential for mitogen-activated protein kinases (MAPK) activities, and involved in regulating growth, apoptosis, and DNA demethylation. In the present study, the cDNA of gcGadd45Ba and gcGadd45Bb in grass carp was identified. And the expression levels show that they were widely distributed in the tested tissues and showed significant immune responses both in vitro and in vivo after challenge with bacteria and pathogen-associated molecular patterns (PAMPs). Overexpression of Gadd45B significantly induced the expression of pro-inflammatory cytokines (IL-1β, IL-8, and TNF-α) and enhanced the phagocytosis activation of grass carp blood cells. These results indicate that Gadd45B plays an important role in innate immune responses.
生长停滞与DNA损伤诱导蛋白45-β（Gadd45B）对于丝裂原活化蛋白激酶（MAPK）的活性至关重要，并参与调节生长、细胞凋亡和DNA去甲基化。在本研究中，鉴定了草鱼中gcGadd45Ba和gcGadd45Bb的cDNA。表达水平表明它们广泛分布于所检测的组织中，并且在用细菌和病原体相关分子模式（PAMP）攻击后，在体外和体内均表现出显著的免疫反应。Gadd45B的过表达显著诱导促炎细胞因子（IL-1β、IL-8和TNF-α）的表达，并增强草鱼血细胞的吞噬激活。这些结果表明Gadd45B在先天免疫反应中起重要作用。
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