Division of Pediatric Dentistry, Department of Orthodontics and Pediatric Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, 50200, Thailand.
Center of Excellence in Oral and Maxillofacial Biology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, 50200, Thailand.
Clin Oral Investig. 2020 Jul;24(7):2363-2374. doi: 10.1007/s00784-019-03095-5. Epub 2019 Dec 14.
To investigate the effects of probiotics, Lactobacillus paracasei SD1, on the quantities of Streptococcus mutans in saliva and plaque samples of preschool children.
This randomized trial recruited 487 preschool children from eight childcare centers. Participants were assigned to receive a 6-month course of placebo milk daily (group I), probiotic milk either daily (group II) or three days a week (triweekly, group III). The absolute quantities of S. mutans and total lactobacilli in the saliva and plaque samples at baseline (T0), after intervention (T6), and 6 months after discontinuation (T12) were assessed by qPCR.
Of 487 children, 354 completed all follow-up periods. However, only 268 children (3.2 ± 0.8 years old; groups I = 86, II = 89, and III = 93) provided adequate saliva for qPCR. Whereas the quantities of S. mutans were significantly decreased in groups II and III compared to group I in the saliva and plaque samples at T6 and T12, those of total lactobacilli were significantly increased (p < 0.0167). There was no difference in the quantities of S. mutans or total lactobacilli between groups II and III at any period. Significant changes in the quantities of S. mutans and total lactobacilli lasted until T12 compared to T0 (p < 0.0167).
Probiotic administration daily or triweekly reduces S. mutans quantities, whereas it increases total lactobacilli quantities that persists at least 6 months after discontinuation in the saliva and plaque samples of preschool children.
Daily or triweekly consumption of L. paracasei SD1 supplemented in milk may help prevent dental caries in preschool children.
研究益生菌干酪乳杆菌 SD1 对学龄前儿童唾液和牙菌斑样本中变异链球菌数量的影响。
本随机试验招募了 8 家日托中心的 487 名学龄前儿童。参与者被分配接受为期 6 个月的安慰剂牛奶（I 组）、益生菌牛奶（每天 II 组或每周 3 天，即每 3 天一次，III 组）。通过 qPCR 评估基线（T0）、干预后（T6）和停止干预后 6 个月（T12）时唾液和牙菌斑样本中 S. mutans 和总乳酸杆菌的绝对数量。
487 名儿童中，354 名完成了所有随访期。然而，只有 268 名儿童（3.2 ± 0.8 岁；I 组 86 名，II 组 89 名，III 组 93 名）提供了足够的唾液进行 qPCR。与 I 组相比，II 组和 III 组的唾液和牙菌斑样本中 S. mutans 的数量在 T6 和 T12 时显著降低，而总乳酸杆菌的数量显著增加（p < 0.0167）。在任何时期，II 组和 III 组之间 S. mutans 和总乳酸杆菌的数量均无差异。与 T0 相比，T6 和 T12 时 S. mutans 和总乳酸杆菌的数量变化均持续存在（p < 0.0167）。
每日或每 3 天一次的益生菌给药可减少 S. mutans 的数量，同时增加总乳酸杆菌的数量，这些数量在停止干预后至少持续 6 个月在学龄前儿童的唾液和牙菌斑样本中存在。
每日或每 3 天一次摄入添加了干酪乳杆菌 SD1 的牛奶可能有助于预防学龄前儿童的龋齿。
