虹鳟鱼肝微粒体（虹鳟）中糖基转移酶的证据。

Evidence for glycosyltransferases in trout liver microsomes (Salmo gairdneri).

Cornillon B, Letoublon R, Frot-Coutaz J, Got R

Laboratoire de Biologie et Technologie des Membranes, Université Claude Bernard-Lyon I, Villeurbanne, France.

Comp Biochem Physiol B. 1979;63(3):419-21. doi: 10.1016/0305-0491(79)90271-2.

Abstract

Trout (Salmo gairdneri) serum is rich in glycoproteins which are synthetized in liver. 2. An attempt to localize glycosyltransferases in hepatocytes is described, using cellular fractionation and marker enzyme determination. 3. Galactosyltransferase, mannosyltransferase, N-acetyl-glucosaminyl transferase, glucosyltransferase, sialyltransferase (on exogenous acceptor) are found in a microsomal fraction obtained by centrifugation at 117 X 10(5) g min of the post-mitochondrial supernatant. 4. Mannose is transferred to endogenous lipids and proteins.

摘要

虹鳟（Salmo gairdneri）血清富含在肝脏中合成的糖蛋白。2. 描述了一项利用细胞分级分离和标记酶测定来定位肝细胞中糖基转移酶的尝试。3. 通过对线粒体后上清液以117×10⁵克·分钟的速度离心获得的微粒体部分中发现了半乳糖基转移酶、甘露糖基转移酶、N - 乙酰葡糖胺基转移酶、葡糖基转移酶、唾液酸基转移酶（以外源受体为底物）。4. 甘露糖被转移到内源性脂质和蛋白质上。