Karaivanova V K, Ivanov S X, Chelibonova-Lorer H
Institute of General and Comparative Pathology, Bulgarian Academy of Sciences, Sofia.
Cancer Biochem Biophys. 1990 Nov;11(4):311-5.
Microsomal sialyltransferase was assayed in chicken liver and hepatoma Mc-29 utilizing liver and hepatoma microsomal glycoprotein fractions, treated with Triton X-100, as exogenous acceptors. In a homologous assay system containing enzyme and acceptor from one and the same tissue no quantitative dependence of enzyme activity was revealed with increasing amount of the acceptor. In mixed experiments in which liver enzyme activity was tested towards hepatoma acceptor glycoproteins, a gradual drop in sialyltransferase activity occurred with increasing quantities of the acceptor. This effect seems to be a consequence of the presence of some inhibitor in the microsomal fractions from the hepatoma cells.
利用经 Triton X - 100 处理的肝和肝癌微粒体糖蛋白组分作为外源性受体，对鸡肝和肝癌 Mc - 29 中的微粒体唾液酸转移酶进行了测定。在一个包含来自同一组织的酶和受体的同源测定系统中，未发现酶活性随受体量增加而存在定量依赖性。在将肝酶活性针对肝癌受体糖蛋白进行测试的混合实验中，随着受体量的增加，唾液酸转移酶活性逐渐下降。这种效应似乎是肝癌细胞微粒体组分中存在某种抑制剂的结果。
