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温度对大鼠和鳟鱼肝脏微粒体中GDP-甘露糖二磷酸多萜醇甘露糖基转移酶体外活性的影响。

Influence of temperature on the in vitro activity of GDP-mannose dolicholphosphate mannosyltransferase in rat and trout liver microsomes.

作者信息

Bizri M, Bouhours J F, Got R

出版信息

Comp Biochem Physiol B. 1985;82(1):157-61. doi: 10.1016/0305-0491(85)90146-4.

Abstract

Temperature optimum of mannosyltransferase activity in liver microsomes is higher in trout than in rat, but this enzymatic activity for rat is higher than trout. Activation energies calculated for mannosyltransferase activity for trout and rat do not correlate with environmental temperature. For a given incubation temperature, Vm values for rat are higher than trout, whereas Km values for trout are lower than rat.

摘要

鳟鱼肝脏微粒体中甘露糖基转移酶活性的最适温度高于大鼠,但大鼠的这种酶活性高于鳟鱼。计算得出的鳟鱼和大鼠甘露糖基转移酶活性的活化能与环境温度无关。在给定的孵育温度下,大鼠的Vm值高于鳟鱼,而鳟鱼的Km值低于大鼠。

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