MoBioFood Research Group, Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, c/Marcel·lí Domingo 1, 43007 Tarragona, Spain.
Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy.
Biomolecules. 2019 Dec 12;9(12):865. doi: 10.3390/biom9120865.
Flavonoids have been shown to modulate in obesity. induces some of its effects through the intestinal receptor (), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.
类黄酮已被证明可以调节肥胖。它通过肠道 G 蛋白偶联受体（）发挥一些作用，尽管目前尚无关于类黄酮如何影响该受体的资料。在这里，我们研究了具有抗肥胖活性的葡萄籽原花青素提取物（GSPE）剂量如何影响肠道，并分析了表观遗传学是否在 GSPE 的长期作用中发挥作用。我们发现， cafeteria 饮食前进行 10 天的 GSPE 处理，可使 GSPE 处理 17 周后回肠中的 mRNA 上调。这与 SP1 转录因子结合区域附近的启动子去甲基化有关。在结肠中，cafeteria 饮食上调了，而没有显示出任何 GSPE 作用。总之，我们已经确定了 GSPE 对回肠中基因表达的长期作用，这些作用部分是通过基因启动子的低甲基化介导的，可能会影响 SP1 结合因子。
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