RNA干扰表明CHOP疗法对犬淋巴瘤有效。
RNA disruption indicates CHOP therapy efficacy in canine lymphoma.
作者信息
Parissenti Amadeo M, Pritzker Laura B, Guo Baoqing, Narendrula Rashmi, Wang Shirly Xiaohui, Lin Lin Laura, Pei Jingchun, Skowronski Karolina, Bienzle Dorothee, Woods J Paul, Pritzker Kenneth P H, Coomber Brenda L
机构信息
Departments of Biology, Chemistry and Biochemistry, Laurentian University, 935 Ramsey Lake Rd., Sudbury, ON, P3E 2C6, Canada.
Rna Diagnostics, c/o Health Sciences North Research Institute, 2nd Floor North, 56 Walford Road, Sudbury, ON, P3E 2H3, Canada.
出版信息
BMC Vet Res. 2019 Dec 16;15(1):453. doi: 10.1186/s12917-019-2189-x.
BACKGROUND
Assessment of the efficacy of a multi-agent chemotherapy protocol in which cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) are administered in canine lymphoma is generally performed by physical measurement of lymph node diameter. However, no consistent correlation has been made with prognostic indicators and the length or absence of clinical remission based on lymph node size. RNA disruption measured mid-therapy has been correlated with increased disease-free survival in recent studies of human cancer and was assessed in this study of canine lymphoma patients. Fine needle aspirate samples were taken before treatment and at weeks 3, 6, and 11 of CHOP therapy. RNA was isolated from these samples and assessed using an Agilent Bioanalyzer. RNA disruption assay (RDA) analysis was performed on the data from the resulting electropherograms.
RESULTS
An increased RNA disruption index (RDI) score was significantly associated with improved progression-free survival.
CONCLUSIONS
Predicting the risk of early relapse during chemotherapy could benefit veterinary patients by reducing ineffective treatment and could allow veterinary oncologists to switch earlier to a more effective drug regimen.
背景
在犬淋巴瘤治疗中，评估环磷酰胺、阿霉素、长春新碱和强的松（CHOP）联合化疗方案的疗效通常是通过物理测量淋巴结直径来进行的。然而，基于淋巴结大小，尚未发现其与预后指标以及临床缓解的时长或有无之间存在一致的相关性。在近期的人类癌症研究中，治疗中期测得的RNA破坏与无病生存期延长相关，本研究对犬淋巴瘤患者进行了此项评估。在治疗前以及CHOP治疗的第3、6和11周采集细针穿刺抽吸样本。从这些样本中分离RNA，并使用安捷伦生物分析仪进行评估。对所得电泳图数据进行RNA破坏分析（RDA）。
结果
RNA破坏指数（RDI）得分增加与无进展生存期改善显著相关。
结论
预测化疗期间早期复发风险可通过减少无效治疗使兽医患者受益，并可让兽医肿瘤学家更早地改用更有效的药物方案。
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