Sierra Matiz Oscar R, Santilli Juliana, Anai Leticia A, Da Silva Maria C L, Sueiro Felipe A, Sequeira Júlio L, Magalhães Larissa F, Magalhães Geórgia M, Tinucci Costa Mirela, Calazans Sabryna G
Clinical Veterinary Department, College of Agricultural and Veterinary Sciences, São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Jaboticabal, São Paulo, Brazil (Sierra Matiz, Anai, Costa, Calazans).
College of Veterinary Medicine, Franca University, Franca, São Paulo, Brazil (Santilli, LF Magalhães).
J Vet Diagn Invest. 2018 Mar;30(2):263-267. doi: 10.1177/1040638717743280. Epub 2017 Dec 1.
Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma in dogs. We evaluated Ki67 immunoexpression and mitotic index (MI) in dogs diagnosed with DLBCL and treated with a 19-wk CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) protocol. Twenty-nine lymph node samples from dogs diagnosed with DLBCL were analyzed for Ki67 immunostaining, and positive cells present in 1 cm were counted in a grid reticle for comparison of survival times above and below the means. The Ki67 mean was 107, and the MI mean was 21. There was a significant ( p < 0.05) difference in median survival time between Ki67 immunostaining above and below the mean, with no difference in MI groups. Ki67 values >107 positive cells per 5 HPF counted in a grid reticle were associated with shorter survival times in dogs with DLBCL treated with a 19-wk CHOP-based protocol.
弥漫性大B细胞淋巴瘤（DLBCL）是犬类中最常见的淋巴瘤类型。我们评估了经19周CHOP（环磷酰胺、阿霉素、长春新碱和泼尼松）方案治疗的确诊为DLBCL的犬的Ki67免疫表达和有丝分裂指数（MI）。对29例确诊为DLBCL的犬的淋巴结样本进行Ki67免疫染色分析，并在网格目镜中计数1平方厘米内的阳性细胞，以比较高于和低于平均值的生存时间。Ki67平均值为107，MI平均值为21。Ki67免疫染色高于和低于平均值的犬之间的中位生存时间存在显著差异（p < 0.05），MI组之间无差异。在经基于19周CHOP方案治疗的DLBCL犬中，网格目镜中每5个高倍视野（HPF）计数的Ki67值>107个阳性细胞与较短的生存时间相关。
