Lalsiamthara Jonathan, Kaur Gurpreet, Gogia Neha, Ali Syed Atif, Goswami Tapas Kumar, Chaudhuri Pallab
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
Biologicals. 2020 Jan;63:62-67. doi: 10.1016/j.biologicals.2019.11.005. Epub 2019 Dec 13.
Brucella abortus S19 is an important tool for controlling bovine brucellosis across the globe. However, vaccination with S19 suffers critical shortcomings such as, presence of residual virulence, induction of abortion and sero-diagnostic interference. In this study, rfbD gene deleted mutant S19 was developed. The mutant strain designated S19ΔR displayed rough LPS phenotype, which was confirmed by acriflavine dye-agglutination and LPS-SDS-PAGE analysis. The virulence was amply reduced as suggested by increased sensitivity to complement killing; reduction in splenic-bacterial load and the recovery time RT50 as validated in mice model. Anti-brucella humoral response was significantly lower as compared to S19 immunization. The minimal induction of Brucella specific IgG1, IgG2a & IgG2b, and IgG3 resulted in no apparent reactivity to RBPT antigen. S19ΔR showed protective index of 1.90 against virulent challenge. S19ΔR being highly attenuated and DIVA compatible may facilitate a platform for developing a safer bovine adulthood vaccine.
布鲁氏菌流产株S19是全球控制牛布鲁氏菌病的重要工具。然而，用S19进行疫苗接种存在严重缺陷，如存在残余毒力、诱导流产和血清学诊断干扰。在本研究中，构建了rfbD基因缺失突变体S19。命名为S19ΔR的突变株表现出粗糙的脂多糖（LPS）表型，这通过吖啶黄染料凝集和LPS-SDS-PAGE分析得到证实。如在小鼠模型中验证的那样，对补体杀伤的敏感性增加、脾脏细菌载量降低以及恢复时间RT50缩短，表明毒力大幅降低。与S19免疫相比，抗布鲁氏菌体液反应显著降低。布鲁氏菌特异性IgG1、IgG2a、IgG2b和IgG3的诱导极少，导致对虎红平板凝集试验（RBPT）抗原无明显反应。S19ΔR对强毒攻击的保护指数为1.90。S19ΔR高度减毒且符合双抗原夹心法（DIVA）原则，可能为开发更安全的牛成年疫苗提供一个平台。
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