Boschiroli M L, Cravero S L, Arese A I, Campos E, Rossetti O L
Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, Moron, Provincia Buenos Aires, Argentina.
Infect Immun. 1997 Feb;65(2):798-800. doi: 10.1128/iai.65.2.798-800.1997.
This study determines whether a genetically engineered mutant of Brucella abortus, strain M-1, possesses differences in protective properties compared to the parental strain, vaccine S19. M-1 is a mutant unable to express BP26, a periplasmic protein with potential use in diagnosis. Mice vaccinated with S19 developed antibodies against BP26, while those vaccinated with M-1 did not. However, mice vaccinated with S19 or M-1 were similarly protected against challenge with pathogenic strain 2308, suggesting that the lack of BP26 does not affect the induction of the protective immune response exerted by S19. These and previous results showing that bacterial invasion and growth or replication in mouse spleens were indistinguishable between strains M-1 and S19 could indicate that the mutant is an attenuated strain which maintains the same protective properties as S19.
本研究旨在确定流产布鲁氏菌的基因工程突变株M-1与亲本菌株疫苗S19相比，在保护特性上是否存在差异。M-1是一种无法表达BP26的突变株，BP26是一种具有潜在诊断用途的周质蛋白。用S19疫苗接种的小鼠产生了针对BP26的抗体，而用M-1疫苗接种的小鼠则没有。然而，用S19或M-1疫苗接种的小鼠在抵抗致病菌株2308的攻击方面受到了类似的保护，这表明缺乏BP26并不影响S19所发挥的保护性免疫反应的诱导。这些结果以及之前的结果表明，M-1和S19菌株在小鼠脾脏中的细菌侵袭、生长或复制没有区别，这可能表明该突变株是一种减毒株，其保持了与S19相同的保护特性。
