Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Regional Campus of International Excellence 'Campus Mare Nostrum', Universidad de Murcia, Murcia, Spain.
Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Regional Campus of International Excellence 'Campus Mare Nostrum', Universidad de Murcia, Murcia, Spain.
Trends Plant Sci. 2020 Feb;25(2):159-175. doi: 10.1016/j.tplants.2019.11.001. Epub 2019 Dec 13.
The discovery of visible fluorescence in the plant pigments betalains revealed the existence of fluorescent patterns in flowers of plants of the order Caryophyllales, where betalains substitute anthocyanins. The serendipitous initial discovery led to a systemized characterization of the role of different substructures on the photophysical phenomenon. Strong fluorescence is general to all members of the family of betaxanthins linked to the structural property that the betalamic acid moiety is connected to an amine group. This property has led to bioinspired tailor-made probes and to the development of novel biotechnological applications in screening techniques or microscopy labeling. Here, we comprehensively review the photophysics, photochemistry, and photobiology of betalain fluorescence and describe all current applications.
甜菜色素中可见荧光的发现揭示了石竹目植物花朵中荧光图案的存在，在这些植物中，甜菜色素取代了花青苷。这一偶然的初步发现促使人们系统地研究了不同亚结构对光物理现象的作用。所有与甜菜醛酸部分与胺基相连的结构特性相关的甜菜酮碱家族成员都具有强烈的荧光。这一特性导致了基于生物启发的定制探针的发展，并在筛选技术或显微镜标记方面开发了新的生物技术应用。在这里，我们全面回顾了甜菜红素荧光的光物理、光化学和光生物学，并描述了所有当前的应用。
