School of Chemical Engineering, Yeungnam University, 280-Daehak-Ro, Gyeongsan 712-749, South Korea.
School of Chemical Engineering, Yeungnam University, 280-Daehak-Ro, Gyeongsan 712-749, South Korea.
Int J Biol Macromol. 2020 Feb 1;144:160-169. doi: 10.1016/j.ijbiomac.2019.12.096. Epub 2019 Dec 13.
Bone tissue engineering aims to design mechanically improved macroporous hydrogels with fibrous topologies using polysaccharides that can provide an appropriate microenvironment in bone defects in order to enhance bone regeneration similar to the native bone extracellular matrix. Herein, we developed hydrogels by intercalation of chitosan (CS) and sodium alginate (SA)-based polyelectrolyte complexes (PECs) (in situ formation using glucuronic acid delta-galactone as an acidifying agent (GDL)) within the poly(acrylamide) (PAM)-crosslinked network (PEC-PAM) during free radical polymerization. The structure and interactions of PEC-PAM were confirmed by FTIR and XRD experiments. The PEC greatly influenced the porosity, pore size, and mechanical properties of hydrogels. Importantly, the PEC within the hydrogels possessed a macroporous structure with a ladder-like fibrous topology, which may provide better cell growth and adhesion. Moreover, the hydrogels showed good bio-mineralization capacity in simulated body fluid solutions as confirmed by FTIR, XRD, FE-SEM, and SEM-EDX. The in vitro performance of the PEC-PAM hydrogels was assessed towards human bone osteoblasts cells in terms of cell proliferation, biocompatibility, and cell adhesion. All of the results suggest that PEC-PAM hydrogels have good potential in bone tissue engineering.
骨组织工程旨在设计机械性能得到改善的具有纤维拓扑结构的大孔水凝胶，使用多糖在骨缺损中提供合适的微环境，以增强类似于天然骨细胞外基质的骨再生。在此，我们通过在自由基聚合过程中在聚（丙烯酰胺）（PAM）交联网络（PEC-PAM）内夹层壳聚糖（CS）和基于海藻酸钠（SA）的聚电解质复合物（PEC）（使用葡萄糖醛酸 delta-半乳糖酮作为酸化剂（GDL）原位形成）来开发水凝胶。通过 FTIR 和 XRD 实验证实了 PEC-PAM 的结构和相互作用。PEC 极大地影响了水凝胶的孔隙率、孔径和机械性能。重要的是，水凝胶中的 PEC 具有具有梯形纤维拓扑结构的大孔结构，这可能为细胞生长和附着提供更好的条件。此外，水凝胶在模拟体液溶液中表现出良好的生物矿化能力，这一点通过 FTIR、XRD、FE-SEM 和 SEM-EDX 得到了证实。通过细胞增殖、生物相容性和细胞附着，评估了 PEC-PAM 水凝胶在人成骨细胞中的体外性能。所有结果均表明 PEC-PAM 水凝胶在骨组织工程中有很好的应用潜力。
