Cancer Biology, Nano Drug Delivery Systems (NDDS), Bio-Innovation Center (BIC), Rajiv Gandhi Centre for Biotechnology, Thycaud P.O, Thiruvananthapuram, Kerala, 695014, India.
Research Scholar, Department of Biotechnology, Faculty of Applied Science & Technology, University of Kerala, Trivandrum, Kerala, 695581, India.
Sci Rep. 2019 Dec 16;9(1):19165. doi: 10.1038/s41598-019-55214-7.
Application of growth factors at wound site has improved the efficiency and quality of healing. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) induce proliferation of various cells in wound healing. Delivery of growth factor from controlled release systems protect it from degradation and also result in sustained delivery of it at the site of injury. The goal of the study was to develop a Polyethylene glycol (PEG) cross-linked cotton-like chitosan scaffold (CS-PEG-H) by freeze-drying method and chemically conjugate heparin to the scaffold to which the growth factors can be electrostatically bound and evaluate its wound healing properties in vitro and in vivo. The growth factor containing scaffolds induced increased proliferation of HaCaT cells, increased neovascularization and collagen formation seen by H and E and Masson's trichrome staining. Immunohistochemistry was performed using the Ki67 marker which increased proliferation of cells in growth factor containing scaffold treated group. Frequent dressing changes are a major deterrent to proper wound healing. Our system was found to release both VEGF and bFGF in a continuous manner and attained stability after 7 days. Thus our system can maintain therapeutic levels of growth factor at the wound bed thereby avoiding the need for daily applications and frequent dressing changes. Thus, it can be a promising candidate for wound healing.
在创伤部位应用生长因子提高了愈合的效率和质量。碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子（VEGF）诱导创伤愈合过程中各种细胞的增殖。从控制释放系统中输送生长因子可以防止其降解，还可以在损伤部位持续输送。该研究的目的是通过冷冻干燥法制备聚乙二醇（PEG）交联棉状壳聚糖支架（CS-PEG-H），并通过化学方法将肝素连接到支架上，生长因子可以通过静电结合到支架上，并评估其在体外和体内的伤口愈合性能。含有生长因子的支架可诱导 HaCaT 细胞增殖增加，通过 H&E 和 Masson 三色染色观察到新生血管形成和胶原形成增加。用 Ki67 标志物进行免疫组织化学染色，增殖细胞增加在生长因子含量较高的支架处理组。频繁更换敷料是妨碍伤口正常愈合的主要原因。我们的系统被发现以连续的方式释放 VEGF 和 bFGF，并在 7 天后达到稳定。因此，我们的系统可以在伤口床维持治疗水平的生长因子，从而避免每日应用和频繁更换敷料的需要。因此，它可能是一种有前途的伤口愈合候选物。
