Department of Bioengineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA; Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA.
Acta Biomater. 2013 Dec;9(12):9351-9. doi: 10.1016/j.actbio.2013.07.030. Epub 2013 Aug 2.
The objective of this research is to develop a dual growth factor-releasing nanoparticle-in-nanofiber system for wound healing applications. In order to mimic and promote the natural healing procedure, chitosan and poly(ethylene oxide) were electrospun into nanofibrous meshes as mimics of extracellular matrix. Vascular endothelial growth factor (VEGF) was loaded within nanofibers to promote angiogenesis in the short term. In addition, platelet-derived growth factor-BB (PDGF-BB) encapsulated poly(lactic-co-glycolic acid) nanoparticles were embedded inside nanofibers to generate a sustained release of PDGF-BB for accelerated tissue regeneration and remodeling. In vitro studies revealed that our nanofibrous composites delivered VEGF quickly and PDGF-BB in a relayed manner, supported fibroblast growth and exhibited anti-bacterial activities. A preliminary in vivo study performed on normal full thickness rat skin wound models demonstrated that nanofiber/nanoparticle scaffolds significantly accelerated the wound healing process by promoting angiogenesis, increasing re-epithelialization and controlling granulation tissue formation. For later stages of healing, evidence also showed quicker collagen deposition and earlier remodeling of the injured site to achieve a faster full regeneration of skin compared to the commercial Hydrofera Blue® wound dressing. These results suggest that our nanoparticle-in-nanofiber system could provide a promising treatment for normal and chronic wound healing.
本研究旨在开发一种用于伤口愈合应用的双生长因子释放纳米粒子-纳米纤维系统。为了模拟和促进自然愈合过程，壳聚糖和聚（氧化乙烯）被电纺成纳米纤维网，作为细胞外基质的模拟物。血管内皮生长因子（VEGF）被加载到纳米纤维内，以在短期内促进血管生成。此外，包封血小板衍生生长因子-BB（PDGF-BB）的聚（乳酸-共-羟基乙酸）纳米颗粒被嵌入纳米纤维内，以产生 PDGF-BB 的持续释放，从而加速组织再生和重塑。体外研究表明，我们的纳米纤维复合材料快速释放 VEGF 和 PDGF-BB，支持成纤维细胞生长，并表现出抗菌活性。在正常全厚大鼠皮肤伤口模型上进行的初步体内研究表明，纳米纤维/纳米颗粒支架通过促进血管生成、增加上皮再形成和控制肉芽组织形成，显著加速了伤口愈合过程。在愈合的后期阶段，证据还表明，与商业 Hydrofera Blue®伤口敷料相比，胶原蛋白沉积更快，损伤部位更早重塑，从而更快地实现皮肤的完全再生。这些结果表明，我们的纳米粒子-纳米纤维系统可为正常和慢性伤口愈合提供一种有前途的治疗方法。
