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留尼汪犬吻蝠微卫星标记多重检测板的开发与特性分析

Development and characterization of a multiplex panel of microsatellite markers for the Reunion free-tailed bat .

作者信息

Dietrich Muriel, Le Minter Gildas, Turpin Magali, Tortosa Pablo

机构信息

UMR PIMIT (Processus Infectieux en Milieu Insulaire Tropical), Université de la Réunion/ INSERM 1187/CNRS 9192/IRD 249, Sainte-Clotilde, Réunion Island.

出版信息

PeerJ. 2019 Dec 12;7:e8036. doi: 10.7717/peerj.8036. eCollection 2019.

Abstract

The ecology and conservation status of many island-restricted bats remain largely unexplored. The free-tailed bat is a small insectivorous tropical bat, endemic to Reunion Island (Indian Ocean). Despite being widely distributed on the island, the fine-scale genetic structure and evolutionary ecology of remain under-investigated, and therefore its ecology is poorly known. Here, we used Illumina paired-end sequencing to develop microsatellite markers for , based on the genotyping of 31 individuals from distinct locations all over the island. We selected and described 12 polymorphic microsatellite loci with high levels of heterozygosity, which provide novel molecular markers for future genetic population-level studies of .

摘要

许多岛屿特有蝙蝠的生态及保护状况在很大程度上仍未得到充分探索。游离尾蝠是一种小型食虫热带蝙蝠,为留尼汪岛(印度洋)所特有。尽管在该岛广泛分布,但其精细尺度的遗传结构和进化生态学仍研究不足,因此对其生态了解甚少。在此,我们基于对该岛不同地点31个个体的基因分型,利用Illumina双末端测序技术为游离尾蝠开发微卫星标记。我们筛选并描述了12个具有高杂合度水平的多态微卫星位点,这些位点为游离尾蝠未来的遗传种群水平研究提供了新的分子标记。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fed/6911689/81014974ac56/peerj-07-8036-g001.jpg

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