UMR PVBMT (Peuplements Végétaux Et Bioagresseurs en Milieu Tropical), Université de La Réunion, Saint-Pierre, La Réunion, France.
UMR PVBMT (Peuplements Végétaux Et Bioagresseurs en Milieu Tropical), Université de La Réunion, Saint-Denis, La Réunion, France.
Mol Biol Rep. 2023 Jun;50(6):5501-5507. doi: 10.1007/s11033-023-08426-0. Epub 2023 Apr 12.
The Manapany day gecko (Phelsuma inexpectata) is endemic to the south of Reunion Island. Threatened by habitat fragmentation and loss, human activities and invasive species, P. inexpectata is considered as critically endangered. Conservation measures are required but data on the species are missing, notably on its genetic diversity and population structure for which no specific markers are available to date. Here, we aimed to develop molecular markers to allow genetic studies of P. inexpectata.
We developed and characterized 20 polymorphic microsatellite markers based on 23 P. inexpectata individuals sampled from 10 sites. Then, the markers were tested on a total of 101 individuals, 30 from a natural site and 71 from an anthropized site. The mean values of N, H and H were 2.3 (± 0.2), 0.353 (± 0.053) and 0.345 (± 0.046) in the natural site and 2.8 (± 0.3), 0.345 (± 0.051) and 0.338 (± 0.048) in the anthropized site, respectively. Based on the combined loci, the probability of identity (PID) for unrelated specimens were 2.7 × 10 and 2.6 × 10 in the natural and anthropized site, respectively.
This work provides the first set of microsatellite markers for P. inexpectata, constituting a valuable tool to conduct classical genetic studies on the species, such as estimating genetic diversity, population structure and kinship relationships among individuals. Such studies will provide relevant information on P. inexpectata and will therefore be helpful in the implementation of conservation measures for this threatened species.
马纳潘尼日行守宫（Phelsuma inexpectata）是留尼汪岛南部的特有物种。由于栖息地破碎化和丧失、人类活动和入侵物种的威胁，P. inexpectata 被认为是极度濒危物种。需要采取保护措施，但目前缺乏关于该物种的信息，特别是关于其遗传多样性和种群结构的信息，因为目前还没有特定的标记物。在这里，我们旨在开发分子标记物，以允许对 P. inexpectata 进行遗传研究。
我们从 10 个地点采集的 23 个 P. inexpectata 个体中开发并表征了 20 个多态性微卫星标记物。然后，在总共 101 个个体中测试了这些标记物，其中 30 个来自自然地点，71 个来自人为地点。在自然地点，N、H 和 H 的平均值分别为 2.3（±0.2）、0.353（±0.053）和 0.345（±0.046），在人为地点，N、H 和 H 的平均值分别为 2.8（±0.3）、0.345（±0.051）和 0.338（±0.048）。基于组合基因座，无关个体的身份识别概率（PID）分别为 2.7×10和 2.6×10，分别在自然和人为地点。
这项工作为 P. inexpectata 提供了第一组微卫星标记物，为该物种进行经典遗传研究提供了有价值的工具，例如估计遗传多样性、种群结构以及个体之间的亲缘关系。这些研究将为 P. inexpectata 提供相关信息，因此有助于实施对这一受威胁物种的保护措施。
