Levin Mykhaylo, Ostanina Natalia, Gumeniuk Oleksii, Meleshko Ruslan, Tereshchenko Oksana, Nikolaieva Yana, Brytsun Vasyl, Tarasenko Nina, Savina Natalia, Kuznetsova Olena, Ocheretiana Natalia, Cheremenko Anatolii, Briazkalo Vadym, Bykov Sergii
State Institution "O.M. Marzieiev Institute for Public Health" NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine.
National Technical University of Ukraine, Igor Sikorsky Kyiv Polytechnic Institute, Faculty of Chemical Engineering, Ukraine.
Heliyon. 2019 Nov 14;5(11):e02748. doi: 10.1016/j.heliyon.2019.e02748. eCollection 2019 Nov.
Fast and simple spectrophotometric method for quantitative determination of mometasone furoate in a single dose (single actuation) of its nasal spray was developed and validated. This method is based on the spectrophotometric analysis of turbid solution of a single spray in 20 mL isopropanol at 220-310 nm. We further show applicability of this method for analysis of large number of single sprays for the dosing homogeneity. It is based on a new approach to the development of spectrophotometric quantitative determination methods for drug products and possibly other objects, allowing measurements to be carried out on sufficiently turbid test solutions. The proposed approach is not a variant of the derivative spectrophotometry and can replace the methods of derivative spectrophotometry in cases where: derivative of analyte spectrum is not intense enough; derivative of the spectrum of the matrix (turbidity and remaining components of the product) is significant when compared with the derivative of the analyte.
开发并验证了一种快速简便的分光光度法，用于定量测定单剂量（单次喷雾）糠酸莫米松鼻喷雾剂中的糠酸莫米松。该方法基于对20 mL异丙醇中单次喷雾的浑浊溶液在220 - 310 nm处进行分光光度分析。我们进一步展示了该方法在分析大量单次喷雾以确定给药均匀性方面的适用性。它基于一种开发药品及可能其他对象的分光光度法定量测定方法的新方法，允许在足够浑浊的测试溶液上进行测量。所提出的方法不是导数分光光度法的变体，并且在以下情况下可以替代导数分光光度法：分析物光谱的导数不够强烈；与分析物的导数相比，基质（产品的浑浊度和其余成分）的光谱导数显著。
