Al-Fotawi Randa, Muthurangan Manikandan, Siyal Abdulaziz, Premnath Sangitha, Al-Fayez Mussad, Mahmood Amer
Department of Oral and Maxillofacial Surgery, Dental Faculty, King Saud University, Riyadh, Saudi Arabia.
Biomed Mater. 2020 Feb 28;15(2):025005. doi: 10.1088/1748-605X/ab6300.
Bone augmentation is a challenging problem in the field of maxillofacial surgery.
In this study, we prepared and evaluated muscle extracellular matrix (MEM) after adding silica calcium phosphate composite (SCPC) seeded with human bone marrow mesenchymal cells (hBMSCs). We then investigated bone augmentation in vivo using the prepared MEM-SCPC.
hBMSCs were seeded on MEM-SCPC, and MEM was characterized. Calvarial bone grafts were prepared using nude mice (n = 12) and grafted separately in two experimental groups: grafts with MEM (control, n = 4) and grafts with MEM-SCPC-hBMSCs (experimental group, n = 8) for 8 weeks. Micro-computed tomography (micro-CT) and histological analysis were then performed.
Micro-CT analysis demonstrated a thinner trabeculae in grafted defects than normal native bone, with a high degree of anisotropy. Quantitative histomorphometric assessment showed a higher median bone percentage surface area of 80.2% ± 6.0% in the experimental group.
The enhanced bone formation and maturation of bone grafted with MEM-SCPC-hBMSCs suggested the potential use of this material for bone augmentation.
骨增量是颌面外科领域中一个具有挑战性的问题。
在本研究中，我们制备并评估了添加接种人骨髓间充质细胞（hBMSCs）的磷酸钙硅复合材料（SCPC）后的肌肉细胞外基质（MEM）。然后我们使用制备好的MEM-SCPC研究其在体内的骨增量情况。
将hBMSCs接种于MEM-SCPC上，并对MEM进行表征。使用裸鼠（n = 12）制备颅骨移植体，并将其分别移植到两个实验组中：MEM移植组（对照组，n = 4）和MEM-SCPC-hBMSCs移植组（实验组，n = 8），持续8周。然后进行微型计算机断层扫描（micro-CT）和组织学分析。
Micro-CT分析显示，移植缺损处的小梁比正常天然骨更薄，各向异性程度较高。定量组织形态计量学评估显示，实验组的中位骨表面积百分比更高，为80.2%±6.0%。
MEM-SCPC-hBMSCs移植骨的骨形成和成熟增强，表明该材料在骨增量方面具有潜在应用价值。
