Yan Yufeng, Tian Xiaobo, Liang Rongguang, Sasian Jose
College of Optical Sciences, 1630 E. University Blvd, Tucson, AZ 85721, USA.
Biomed Opt Express. 2019 Nov 4;10(12):6029-6042. doi: 10.1364/BOE.10.006029. eCollection 2019 Dec 1.
Recently, the development of motion-free 3D microscopy utilizing focus tunable lenses (FTL) has been rapid. However, the downgrade of optical performance due to FTL and its gravity effect are rarely discussed in detail. Also, color dispersion is usually maintained purely depending on the FTL material without further correction. In this manuscript, we provide a quantitative evaluation of the impact of FTL on the optical performance of the microscope. The evaluation is based on both optical ray tracing simulations and lab experiments. In addition, we derive the first order conditions to correct axial color aberration of FTL through its entire power tuning range. Secondary spectrum correction is also possible and an apochromatic motion-free 3D microscope with 2 additional doublets is demonstrated. This study will serve a guidance in utilizing FTL as a motion-free element for 3D microscopy.
近年来，利用聚焦可调透镜（FTL）的无运动3D显微镜发展迅速。然而，FTL导致的光学性能下降及其重力效应鲜有详细讨论。此外，色散通常仅依靠FTL材料维持，未作进一步校正。在本论文中，我们对FTL对显微镜光学性能的影响进行了定量评估。该评估基于光线追迹模拟和实验室实验。此外，我们推导了在FTL整个焦度调谐范围内校正轴向色差的一阶条件。二次光谱校正也是可行的，并且展示了一款带有两个附加双合透镜的复消色差无运动3D显微镜。本研究将为利用FTL作为3D显微镜的无运动元件提供指导。
