Centre for Advanced Imaging, and Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, QLD, 4072, Australia.
Central Analytical Research Facility, Institute for Future Environments, Queensland University of Technology, Brisbane, QLD, 4001, Australia.
Angew Chem Int Ed Engl. 2020 Mar 2;59(10):3855-3858. doi: 10.1002/anie.201911390. Epub 2020 Jan 23.
Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High-resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high-resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ.
酶是大多数生理过程的核心组成部分，因此与各种病理有关。因此，组织内酶活性的高分辨率图谱代表了阐明健康和疾病中酶功能的有力工具。在这里，我们提出了一种新的质谱成像（MSI）方法，可直接从组织中测定酶活性的空间分布。对暴露于磷脂底物的组织切片进行 MSI 分析，可生成高分辨率的磷脂酶活性和特异性图谱，随后可与同一切片的组织学图像进行比较。因此，功能 MSI 代表了一种新的、可推广的原位生物活性成像方法。
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