Zhao Qing, Liu Meng-Ying, Lü Hui, Liang Jun-Yu, Diao Xing-Xing, Zhang Xin, Meng Liao
School of Civil Engineering, Guangzhou University, Guangzhou 510006, China.
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Huan Jing Ke Xue. 2019 Sep 8;40(9):4195-4201. doi: 10.13227/j.hjkx.201901247.
In order to upgrade the current shortcut nitrification and denitrification process for landfill leachate treatment and to stimulate nitrification-denitrification coupled with anaerobic ammonia oxidation (ANAMMOX) at a landfill, an ANAMMOX process was started using an up-flow anaerobic sludge bed (UASB) reactor seeded with nitrification and denitrification sludge. The performances of the reactor were investigated, including the nitrogen loading and nitrogen removal rates. Moreover, Illumina Miseq sequencing was conducted to analyze the microbial community dynamics under long-term operation on a molecular level. The results showed that the ANAMMOX reactor was successfully started in 149 days. The total nitrogen loading rate reached 4000.00 mg·(L·d), and the total nitrogen removal rate reached 3885.76 mg·(L·d) after stable operation. The average ammonium and nitrite removal efficiencies were more than 95%. In 250 days, the Planctomycetes in the reactor experienced rapid growth, and its abundance reached 54.94%. The abundance of reached 49.66%. The upgrading process of landfill leachate treatment by coupling ANAMMOX based on short-cut nitrification and denitrification was confirmed to be feasible.
为了升级当前用于垃圾渗滤液处理的短程硝化反硝化工艺，并在垃圾填埋场促进硝化反硝化与厌氧氨氧化（ANAMMOX）耦合，采用接种了硝化和反硝化污泥的上流式厌氧污泥床（UASB）反应器启动了ANAMMOX工艺。研究了该反应器的性能，包括氮负荷和氮去除率。此外，进行了Illumina Miseq测序，以在分子水平上分析长期运行下的微生物群落动态。结果表明，ANAMMOX反应器在149天内成功启动。稳定运行后，总氮负荷率达到4000.00 mg·(L·d)，总氮去除率达到3885.76 mg·(L·d)。铵和亚硝酸盐的平均去除效率超过95%。在250天内，反应器中的浮霉菌经历了快速生长，其丰度达到54.94%。 的丰度达到49.66%。基于短程硝化反硝化耦合ANAMMOX的垃圾渗滤液处理升级工艺被证实是可行的。
