Key Laboratory of Urban Stormwater System and Water Environment (Ministry of Education), Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Key Laboratory of Urban Stormwater System and Water Environment (Ministry of Education), Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Environ Res. 2020 Dec;191:110131. doi: 10.1016/j.envres.2020.110131. Epub 2020 Aug 28.
The current research regarding anaerobic ammonium oxidation (anammox) for the treatment of landfill leachate mainly focuses on a temperature range of 30-35 °C. However, achieving and maintaining anammox at lower temperatures would widen its application for the treatment of landfill leachate. This study, attempts to address this issue by using a combined process involving an upflow anaerobic sludge blanket (UASB), anoxic/oxic (A/O) reactor, anammox reactor (ANAOR), and anaerobic sequencing batch reactor (ASBR) to enrich anammox bacteria at relatively low temperatures. The rapid start-up of the partial nitrification-anammox process for landfill leachate treatment was achieved and maintained at 13-22 °C. The experiment was divided into phase 1 (20-22 °C) and phase 2 (13-15 °C). The results showed that 87.1% of the chemical oxygen demand (COD), 97.4-97.7% of the ammonium nitrogen (NH-N), and 93.3-94.7% of the total nitrogen (TN), were removed. At least 29.3% and 11.4% of NH-N was removed through anammox in phases 1 and 2, respectively, with an accumulation NO-N ratio of 86.1-88.6%. Candidatus Kuenenia was the dominant anammox bacteria in the anammox process. A low temperature of 13-15 °C did not affect ammonia oxidizing bacteria (AOB), and their relative abundance in the A/O reactor ranged from 27.29% to 33.22%.
目前关于用于处理垃圾渗滤液的厌氧氨氧化（anammox）的研究主要集中在 30-35°C 的温度范围内。然而，在较低温度下实现和维持 anammox 将拓宽其在处理垃圾渗滤液方面的应用。本研究试图通过使用上流式厌氧污泥床（UASB）、缺氧/好氧（A/O）反应器、厌氧氨氧化反应器（ANAOR）和厌氧序批式反应器（ASBR）的组合工艺来解决这个问题，在相对较低的温度下富集厌氧氨氧化菌。成功实现并维持了 13-22°C 下垃圾渗滤液处理的部分硝化-厌氧氨氧化工艺的快速启动。实验分为第 1 阶段（20-22°C）和第 2 阶段（13-15°C）。结果表明，化学需氧量（COD）的去除率为 87.1%，氨氮（NH-N）的去除率为 97.4-97.7%，总氮（TN）的去除率为 93.3-94.7%。第 1 阶段和第 2 阶段分别至少通过厌氧氨氧化去除了 29.3%和 11.4%的 NH-N，积累的 NO-N 比为 86.1-88.6%。厌氧氨氧化过程中的优势厌氧氨氧化菌为卡氏菌属（Candidatus Kuenenia）。13-15°C 的低温不会影响氨氧化菌（AOB），它们在 A/O 反应器中的相对丰度在 27.29%至 33.22%之间。
