College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Gen Comp Endocrinol. 2020 Mar 1;288:113371. doi: 10.1016/j.ygcen.2019.113371. Epub 2019 Dec 16.
Mammalian bombesin-related peptide, neuromedin B (NMB) action is mediated by its receptor (NMBR), and NMB/NMBR system plays a major role in regulating hormone secretions, reproduction and cell growth. Here we report the functions of NMB in regulating steroidogenesis (testosterone synthesis), cell viability and apoptosis. The primary rabbit Leydig cells were employed as the paradigm for this research. We initially confirmed that NMBR is distributed in Leydig cells of rabbit testis, and a certain dose of NMB could increase the secretion of testosterone in primary cultured rabbit Leydig cells. Subsequently, the accumulated NMBR, StAR, CYP11A1, 3β-HSD and PKC protein could be induced by a certain dose of NMB in Leydig cells. Moreover, we found that NMB could decrease the cell viability, and decreased the expression of PCNA protein in Leydig cells; meanwhile, except for 100 nM, other doses of NMB could suppress the cell apoptosis, and regulate Caspase-3 protein expression in Leydig cells, respectively. These results identify that NMB may be a key factor in regulating testosterone synthesis through taking part in NMBR/PKC/steroidogenesis signaling pathway, as well as the cell viability and proliferation in rabbit Leydig cells.
哺乳动物蛙皮素相关肽，神经介素 B（NMB）的作用是由其受体（NMBR）介导的，NMB/NMBR 系统在调节激素分泌、生殖和细胞生长方面起着重要作用。在这里，我们报告了 NMB 在调节类固醇生成（睾丸素合成）、细胞活力和细胞凋亡中的作用。我们最初证实 NMBR 分布在兔睾丸的 Leydig 细胞中，一定剂量的 NMB 可以增加原代培养的兔 Leydig 细胞中睾丸素的分泌。随后，一定剂量的 NMB 可以在 Leydig 细胞中诱导积累 NMBR、StAR、CYP11A1、3β-HSD 和 PKC 蛋白。此外，我们发现 NMB 可以降低 Leydig 细胞的活力，并降低 PCNA 蛋白的表达；同时，除了 100 nM 以外，其他剂量的 NMB 可以分别抑制 Leydig 细胞的细胞凋亡，并调节 Caspase-3 蛋白的表达。这些结果表明，NMB 可能是通过参与 NMBR/PKC/类固醇生成信号通路以及兔 Leydig 细胞的细胞活力和增殖来调节睾丸素合成的关键因素。
