Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China.
Department of Otolaryngology, Head and Neck Surgery, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou 362000, China.
Biosensors (Basel). 2023 Feb 8;13(2):241. doi: 10.3390/bios13020241.
The rapid development of personalized medicine places high demands on the control of drug dose and cellular drug response to provide patients with better curative effects and low side effects. To solve the problem of low detection accuracies of the cell-counting kit-8 (CCK8) method, a detection method based on surface-enhanced Raman spectroscopy (SERS) of cell-secreted proteins was adopted to evaluate the concentration of the anticancer drug cisplatin and the cellular drug response of nasopharyngeal carcinoma. CNE1 and NP69 cell lines were used to evaluate cisplatin response. The results showed that the combination of the SERS spectrum with principal component analysis-linear discriminant analysis could detect the difference in the response of cisplatin with a concentration difference of 1 μg/mL, which considerably exceeded that of CCK8. In addition, the SERS spectral peak intensity of the cell-secreted proteins strongly correlated with the cisplatin concentration. Furthermore, the mass spectrum of the secreted proteins of the nasopharyngeal carcinoma cells was analyzed to verify the results obtained using the SERS spectrum. The results demonstrated that SERS of secreted proteins has great potential for high-precision detection of chemotherapeutic drug response.
个性化医学的快速发展对药物剂量控制和细胞药物反应提出了很高的要求，以提供更好的疗效和低副作用。为了解决细胞计数试剂盒-8（CCK8）方法检测精度低的问题，采用基于细胞分泌蛋白的表面增强拉曼光谱（SERS）的检测方法来评估抗癌药物顺铂的浓度和鼻咽癌的细胞药物反应。使用 CNE1 和 NP69 细胞系评估顺铂的反应。结果表明，SERS 光谱与主成分分析-线性判别分析相结合，能够检测出浓度差为 1μg/mL 的顺铂反应差异，明显超过 CCK8。此外，细胞分泌蛋白的 SERS 光谱峰强度与顺铂浓度强烈相关。此外，还分析了鼻咽癌细胞分泌蛋白的质谱，以验证 SERS 光谱获得的结果。结果表明，分泌蛋白的 SERS 具有高精度检测化疗药物反应的巨大潜力。
