从蛤蜊中分离得到一种具有强抗凝和纤溶活性的肝素类似物的分离与表征。

Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam .

机构信息

School of Food Science and Technology, Guangdong Ocean University, Zhan Jiang 524088, China.

Shenzhen institute, Guangdong Ocean University, Shen Zhen 518108, China.

出版信息

Mar Drugs. 2019 Dec 19;18(1):6. doi: 10.3390/md18010006.

DOI:10.3390/md18010006

PMID:31861572

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7024239/

Abstract

Heparin from mollusks with unique sulfated glycosaminoglycan exhibits strong anti-thrombotic activities. This study reports on a purified heparinoid from , which shows potent anticoagulant and fibrinolytic abilities. Its structure was characterized by infrared spectroscopy, high-performance liquid chromatography, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. Its fibrinolytic activity was determined in vitro and in vivo. Its anticoagulant activity was determined by activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicated that clam heparinoid was a homogeneous glycosaminoglycan with a molecular weight of 30.99 kDa, mainly composed of →4)-α-IdoA2S-(1→4)-α-GlcNS3S6S (or GlcNS6S)-(1→4)-β-GlcA-(1→4)-α-GlcNS6S (or GlcNAC)-(1→. Furthermore, this heparinoid showed a highly anticoagulant titer and fibrinolytic value of 149.63 IU/mg and 1.96 IU/mg, respectively. In summary, clam heparinoid shows great potential for application in the clinic and antithrombotic drugs industry.

摘要

从贝类中提取的具有独特硫酸化糖胺聚糖的肝素具有很强的抗血栓活性。本研究报道了一种从贻贝中提取的肝素类似物，它具有很强的抗凝和纤溶能力。其结构通过红外光谱、高效液相色谱、一维和二维核磁共振波谱进行了表征。其纤溶活性在体外和体内进行了测定。其抗凝活性通过活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）和凝血时间（TT）来测定。结果表明，贻贝肝素类似物是一种均一的糖胺聚糖，分子量为 30.99 kDa，主要由→4)-α-IdoA2S-(1→4)-α-GlcNS3S6S（或 GlcNS6S）-(1→4)-β-GlcA-(1→4)-α-GlcNS6S（或 GlcNAC）-(1→组成。此外，该肝素类似物的抗凝效价和纤溶值分别高达 149.63 IU/mg 和 1.96 IU/mg。总之，贻贝肝素类似物在临床和抗血栓药物工业中有很大的应用潜力。