Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China.
Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China.
Int J Biol Macromol. 2018 Mar;108:710-718. doi: 10.1016/j.ijbiomac.2017.11.058. Epub 2017 Nov 22.
In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra. We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,H andC NMR spectra and methylation analysis. According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone. The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29. The anticoagulant activities of the HsG was evaluated and compared with heparin. The result showed that the HsG could prolong the activated partial thromboplastin time.
在这项研究中，从海参 Holothuria scabra 中分离出一种平均分子量为 69.1 kDa 的新岩藻糖基硫酸软骨素（HsG）。我们通过单糖组成分析、二糖组成分析、IR、H 和 C NMR 光谱以及甲基化分析来研究 HsG 的结构。根据脱硫/羧基还原多糖的甲基化结果和脱岩藻糖基多糖的酶解生成的不饱和二糖分析，表明每个支链由一个岩藻糖吡喃基残基形成，其中 55.7%的岩藻糖吡喃基残基连接到 N-乙酰半乳糖胺部分的 O-6 位，21.2%的岩藻糖吡喃基残基连接到 β-d-葡萄糖醛酸的 O-3 位，13.0%的岩藻糖吡喃基残基连接到 N-乙酰半乳糖胺部分的 O-4 位，10.1%的岩藻糖吡喃基残基不与主链上的硫酸基连接。主链→4）GlcUAβ（1→3）GalNAcβ（1→和硫酸岩藻糖支链由 β-d-葡萄糖醛酸、N-乙酰-β-d-半乳糖胺、α-l-岩藻糖和硫酸根组成，摩尔比为 1:1.72:2.34:3.29。评估了 HsG 的抗凝活性并与肝素进行了比较。结果表明，HsG 可以延长活化部分凝血活酶时间。
