Rodríguez-Andrade Ernesto, Stchigel Alberto M, Guarro Josep, Cano-Lira José F
Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21, 43201 Reus, Tarragona, Spain.
Microorganisms. 2019 Dec 19;8(1):12. doi: 10.3390/microorganisms8010012.
Filamentous fungi are rarely reported as responsible for spoiling wine. Cork taint was detected in sparkling wine; therefore, we investigated fungal contamination as a possible cause of organoleptic alteration. Spoiled wine was filtered and membranes were plated onto potato dextrose agar (PDA). The cork stoppers used for sealing bottles were cut and also plated onto PDA. Fungal strains were phenotypically characterized and molecularly identified by sequencing of a fragment of the 28S nrRNA gene (LSU) and (occasionally) by other additional molecular markers. Twenty-seven strains were isolated and sixteen species were identified, all of them belonging to the phylum Ascomycota. The fungi isolated from wine were three species of section , a species of section and . was isolated from both sort of samples, and the fungi isolated from cork stoppers were and . Surprisingly, most of the taxa recovered from the cork stoppers and/or wine were new to the science: a new genus () and seven new species belonging to the genera , , , , and . Future studies could let us know if these fungi would be able to produce compounds responsible for cork taint.
丝状真菌很少被报道为葡萄酒变质的原因。在起泡酒中检测到软木塞污染；因此，我们调查了真菌污染作为感官改变的可能原因。对变质葡萄酒进行过滤，并将滤膜接种到马铃薯葡萄糖琼脂（PDA）上。将用于密封瓶子的软木塞切割后也接种到PDA上。通过对28S nrRNA基因（LSU）片段进行测序，对真菌菌株进行表型特征分析和分子鉴定，（偶尔）还使用其他额外的分子标记。共分离出27株菌株，鉴定出16个物种，它们均属于子囊菌门。从葡萄酒中分离出的真菌有三个种属于 组，一个种属于 组和 组。 从两种样品中均有分离出，从软木塞中分离出的真菌有 和 。令人惊讶的是，从软木塞和/或葡萄酒中分离出的大多数分类单元对科学界来说都是新的：一个新属（ ）以及属于 、 、 、 和 属的七个新物种。未来的研究可以让我们了解这些真菌是否能够产生导致软木塞污染的化合物。
