Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, 253 Gongye Street, Haizhu, Guangzhou, 510280, China.
Stem Cell Rev Rep. 2019 Dec;15(6):774-784. doi: 10.1007/s12015-019-09909-z.
The current understanding and effective treatment of liver disease is far from satisfactory. Liver organoids and liver buds (LBs) transforming cell culture from two dimensions(2D) to three dimensions(3D) has provided infinite possibilities for stem cells to use in clinic. Recent technological advances in the 3D culture have shown the potentiality of liver organoids and LBs as the promising tool to model in vitro liver diseases. The induced LBs and liver organoids provide a platform for cell-based therapy, liver disease models, liver organogenesis and drugs screening. And its genetic heterogeneity supplies a way for the realization of precision medicine.
目前，对肝脏疾病的认识和有效治疗还远远不能令人满意。将细胞培养从二维（2D）转变为三维（3D）的肝类器官和肝芽（LBs）为干细胞在临床上的应用提供了无限的可能性。最近 3D 培养技术的进步显示了肝类器官和 LBs 作为体外肝脏疾病模型的有前途的工具的潜力。诱导的 LBs 和肝类器官为基于细胞的治疗、肝脏疾病模型、肝脏发生和药物筛选提供了一个平台。其遗传异质性为实现精准医学提供了一种方法。
