Ochi Kenta, Okamoto Mariko, Okamoto Misaki, Okura Masatoshi, Takamatsu Daisuke
Ehime Prefectural Livestock Disease Diagnostic Center, Toon, Ehime 791-0212, Japan.
Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci. 2020 Feb 4;82(2):109-114. doi: 10.1292/jvms.19-0522. Epub 2019 Dec 20.
Trueperella pyogenes is an opportunistic pathogen that causes a wide variety of purulent infections. We recently isolated a T. pyogenes strain unable to be identified by the previously reported T. pyogenes pyolysin gene (plo)-specific PCR from the lung of a sheep with astasia. Sequence comparison of plo among representative strains revealed several nucleotide substitutions in the primer-annealing regions. As such substitutions were considered to be a reason for the low PCR specificity, we designed novel primers in conserved regions of plo. Under optimized conditions, the novel primers precisely identified all T. pyogenes strains tested, and no products were generated from any other bacterial strains, suggesting the usefulness of the novel PCR assay for the diagnosis of T. pyogenes infections.
化脓放线菌是一种引起多种化脓性感染的机会致病菌。我们最近从一只患有起立不能症的绵羊的肺中分离出一株化脓放线菌，该菌株无法通过先前报道的化脓放线菌溶血素基因（plo）特异性PCR进行鉴定。代表性菌株中plo的序列比较显示引物退火区域存在几个核苷酸替换。由于这些替换被认为是PCR特异性低的原因，我们在plo的保守区域设计了新的引物。在优化条件下，新引物准确鉴定了所有测试的化脓放线菌菌株，并且没有从任何其他细菌菌株产生产物，这表明新的PCR检测方法对化脓放线菌感染诊断的有用性。
