参与杆菌硫醇生物合成的锌依赖性脱乙酰酶BshB的X射线晶体结构。
X-ray crystallographic structure of BshB, the zinc-dependent deacetylase involved in bacillithiol biosynthesis.
作者信息
Woodward Robert L, Castleman Michaela M, Meloche Chelsea E, Karpen Mary E, Carlson Clare G, Yobi William H, Jepsen Jacqueline C, Lewis Benjamin W, Zarnosky Brooke N, Cook Paul D
机构信息
Department of Chemistry and Biochemistry, University of Mount Union, Alliance, Ohio.
Department of Chemistry, Grand Valley State University, Allendale, Michigan.
出版信息
Protein Sci. 2020 Apr;29(4):1035-1039. doi: 10.1002/pro.3808. Epub 2019 Dec 31.
Many gram-positive bacteria produce bacillithiol to aid in the maintenance of redox homeostasis and degradation of toxic compounds, including the antibiotic fosfomycin. Bacillithiol is produced via a three-enzyme pathway that includes the action of the zinc-dependent deacetylase BshB. Previous studies identified conserved aspartate and histidine residues within the active site that are involved in metal binding and catalysis, but the enzymatic mechanism is not fully understood. Here we report two X-ray crystallographic structures of BshB from Bacillus subtilis that provide insight into the BshB catalytic mechanism.
许多革兰氏阳性菌会产生杆菌硫醇，以帮助维持氧化还原稳态并降解包括抗生素磷霉素在内的有毒化合物。杆菌硫醇通过一条三酶途径产生，该途径包括锌依赖性脱乙酰酶BshB的作用。先前的研究确定了活性位点内参与金属结合和催化的保守天冬氨酸和组氨酸残基，但酶促机制尚未完全了解。在此，我们报告了来自枯草芽孢杆菌的BshB的两个X射线晶体结构，这些结构为BshB催化机制提供了深入见解。
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