Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, 102206, China.
Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing University of Agriculture, Beijing, 102206, China.
Plant Physiol Biochem. 2020 Feb;147:215-222. doi: 10.1016/j.plaphy.2019.12.019. Epub 2019 Dec 17.
Ethylene-response factor (ERF) proteins are members of a transcription factor family involved in plant growth and environmental stress responses, but the biological functions of ERF members in adzuki bean (Vigna angularis var. angularis) remain unknown. In addition, it is unclear whether these proteins have a role in regulating responses to abiotic stressors. Here, we identified 47 ERF genes by analyzing the adzuki bean genome. Whole-transcriptome analyses of plants under saline-alkaline stress suggested that the expression of 13 ERF genes was induced in response to saline-alkaline stress. Analysis of the cis-acting elements showed that the promoters of these saline-alkaline stress-inducible ERF genes contained LTRs, DREs, MYBs, ABREs, MYCs, CGTCA-, and TGACG-motifs, which are involved in abiotic stress responses. The expression of VaERF3 was induced by NaHCO, polyethylene glycol 6000, NaCl, and ABA (abscisic acid), as determined by qRT-PCR. Overexpression of VaERF3 in transgenic Arabidopsis resulted in higher levels of proline accumulation and lower malondialdehyde and reactive oxygen species contents in plants grown under saline-alkaline stress conditions. Moreover, VaERF3 encoded a nuclear-localized transcriptional activator that promoted the expression of stress-responsive genes. Collectively, these results are of great significance in elucidating the mechanisms of saline-alkaline stress responses in adzuki bean.
乙烯响应因子（ERF）蛋白是参与植物生长和环境胁迫响应的转录因子家族的成员，但小豆（Vigna angularis var. angularis）中 ERF 成员的生物学功能尚不清楚。此外，这些蛋白质是否在调节对非生物胁迫因子的响应中发挥作用也不清楚。在这里，我们通过分析小豆基因组鉴定了 47 个 ERF 基因。盐碱性胁迫下植物的全转录组分析表明，13 个 ERF 基因的表达受到盐碱性胁迫的诱导。顺式作用元件分析表明，这些盐碱性胁迫诱导的 ERF 基因的启动子含有 LTRs、DREs、MYBs、ABREs、MYCs、CGTCA-和 TGACG 基序，这些基序参与非生物胁迫响应。通过 qRT-PCR 测定，VaERF3 的表达受 NaHCO、聚乙二醇 6000、NaCl 和 ABA（脱落酸）诱导。在转基因拟南芥中过表达 VaERF3 导致在盐碱性胁迫条件下生长的植物中脯氨酸积累水平升高，丙二醛和活性氧物质含量降低。此外，VaERF3 编码一种核定位的转录激活子，可促进应激响应基因的表达。总之，这些结果对于阐明小豆盐碱性胁迫响应的机制具有重要意义。
