Koochek Dezfuli Mohammad, Seyedmajidi Maryam, Nafarzadeh Shima, Yazdani Farzad, Bijani Ali
Department of Oral and Maxillofacial Pathology, Mazandaran University of Medical Sciences, Sari, Iran.
Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Asian Pac J Cancer Prev. 2019 Dec 1;20(12):3547-3553. doi: 10.31557/APJCP.2019.20.12.3547.
This study aimed to investigate the immunohistochemical expression of CD31 and podoplanin in order to examine angiogenesis and lymphangiogenesis, respectively in common malignant tumors of salivary glands.
Forty formalin-fixed, paraffinated blocks (20 adenoid cystic carcinoma and 20 mucoepidermoid carcinoma blocks) were selected from the medical archives of Amir A'lam Hospital of Tehran, Iran. Sections from the blocks were stained by CD31 and D2-40 markers via immunohistochemistry. Clinical and demographic information was extracted from the patients' records.
There was a significant difference between tumors in terms of intratumoral microvessel density (MVD) (P< 0.001), total MVD (P< 0.001), and intratumoral lymphatic vessel density (LVD) (P= 0.011). In mucoepidermoid carcinoma, intratumoral MVD and LVD were greater than peritumoral MVD and LVD (P= 0.001 and P< 0.001, respectively). In mucoepidermoid carcinoma, there was no relationship between histological grade with MVD (total, intratumoral or peritumoral) or LVD (total, intratumoral or peritumoral) (P> 0.05). A similar finding was reported with respect to the histopathological grade of adenoid cystic carcinoma (P> 0.05).
The higher level of angiogenesis and lymphangiogenesis in mucoepidermoid carcinoma, specifically at the center of tumor, compared to adenoid cystic carcinoma, may be attributed to differences in the clinical behaviors and metastasis of tumors. Moreover, considering the high LVD at the center of tumor in mucoepidermoid carcinoma and infrequency of metastasis to regional lymph nodes in adenoid cystic carcinoma, it can play a significant role in metastasis to regional lymph nodes.
本研究旨在调查CD31和血小板内皮细胞黏附分子-1的免疫组化表达，以便分别检测涎腺常见恶性肿瘤中的血管生成和淋巴管生成情况。
从伊朗德黑兰阿米尔·阿拉姆医院的医学档案中选取40个福尔马林固定、石蜡包埋的组织块（20个腺样囊性癌组织块和20个黏液表皮样癌组织块）。通过免疫组化法用CD31和D2-40标记物对组织块切片进行染色。从患者记录中提取临床和人口统计学信息。
肿瘤在瘤内微血管密度（MVD）（P<0.001）、总MVD（P<0.001）和瘤内淋巴管密度（LVD）（P=0.011）方面存在显著差异。在黏液表皮样癌中，瘤内MVD和LVD大于瘤周MVD和LVD（分别为P=0.001和P<0.001）。在黏液表皮样癌中，组织学分级与MVD（总、瘤内或瘤周）或LVD（总、瘤内或瘤周）之间无相关性（P>0.05）。腺样囊性癌的组织病理学分级也有类似结果（P>0.05）。
与腺样囊性癌相比，黏液表皮样癌中血管生成和淋巴管生成水平较高，特别是在肿瘤中心，这可能归因于肿瘤临床行为和转移的差异。此外，考虑到黏液表皮样癌肿瘤中心的高LVD以及腺样囊性癌区域淋巴结转移不常见，LVD在区域淋巴结转移中可能起重要作用。
