College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China.
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China.
Environ Pollut. 2020 Mar;258:113827. doi: 10.1016/j.envpol.2019.113827. Epub 2019 Dec 17.
Polycyclic aromatic hydrocarbons (PAHs) pollution has become a worldwide environmental problem, and the spread of PAHs can cause carcinogenicity, mutagenicity, and toxicity to humans. However, the transfer and accumulation of PAHs in crop leaves has not been clearly understood. In this study, we first reported that the environmental temperature could induce phenanthrene transfer and accumulation in hydrocultured wheat, corn and soybean leaves via vacuum-infiltration-centrifugation method. Phenanthrene accumulation rises significantly (p < 0.05) in the first 8 h and reaches the maximum accumulation rate at the 4th h. Then the accumulation turns stable in both apoplast and sympalst of wheat, soybean and corn leaves. Temperature is positively correlated with phenanthrene accumulation in apoplast and sympalst of soybean and corn leaves, and phenanthrene accumulation increases under lower temperature in apoplast and sympalst of wheat leaves. Temperature also displays a positive correlation with the phenanthrene accumulation under gradient phenanthrene treatments in both apoplast and sympalst. In addition, the wheat, corn and soybean leaves have the same phenanthrene accumulation pathways and symplast pathway is major for phenanthrene accumulation with the contribution over 55% in total phenanthrene accumulation. Meanwhile, based on the Elovich equation, the symplast and apoplast processes of phenanthrene accumulation are endothermic. In the end, our findings will offer a new understanding for phenanthrene transfer and accumulation pathway in plant leaves and put forward a new biological reference of PAHs transfer in environmental science.
多环芳烃（PAHs）污染已成为全球性的环境问题，PAHs 的传播可对人类产生致癌性、致突变性和毒性。然而，PAHs 在作物叶片中的迁移和积累还没有被清楚地了解。在本研究中，我们首次通过真空渗透离心法报道了环境温度可以通过真空渗透离心法诱导水培小麦、玉米和大豆叶片中菲的迁移和积累。菲的积累在 8 h 内显著升高（p < 0.05），并在第 4 h 达到最大积累率。然后，菲在小麦、大豆和玉米叶片的质外体和共质体中达到稳定积累。温度与大豆和玉米叶片的质外体和共质体中菲的积累呈正相关，低温下小麦叶片质外体和共质体中的菲积累增加。温度也与质外体和共质体中梯度菲处理下的菲积累呈正相关。此外，小麦、玉米和大豆叶片具有相同的菲积累途径，共质体途径是菲积累的主要途径，在总菲积累中贡献超过 55%。同时，基于 Elovich 方程，菲在质外体和共质体中的积累过程是吸热的。最后，我们的研究结果将为植物叶片中菲的迁移和积累途径提供新的认识，并为环境科学中 PAHs 的迁移提供新的生物学参考。
