Huo Jiao, Liu Yunjie, Zeng Zhu, Zhu Xuejiao, Peng Zihao, Chen Jinyao, Zhang Lishi
West China School of Public Health, Sichuan University, Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2019 Nov;48(6):976-1000.
This study was designed to determine the genotoxicity of 2-methylfuran based on a multi-endpoint genotoxicity test system.
The SPF-grade male SD rats(n = 30) were randomized to six treatment groups, i. e. 4 treatment groups(25, 50, 100 and 150 mg/kg), a control group(vegetable oil) and a positive groups(N-ethyl-N-nitrosourea, 80 mg/kg). All treatments were administrated by gavage for continuous 3 days. Tail vein blood for comet assay was collected at 3 h after the final administration. Pig-a gene mutation assays were performed on days 0(one day before gavage), 14 and 28. Micronucleus tests in peripheral blood using flow cytometry were performed on days 0 and 4.
A statistically significant increase in tail intensity was observed at 150 mg/kg for peripheral blood in comet assay. There was no significant difference among the groups in mutant cell frequency of erythrocytes and reticulocytes at 2 timepoints in Pig-a gene mutation assay, and no significant difference among the groups in the frequency of micronucleus in micronucleus test.
The result of genotoxicity tests suggested that 2-methylfuran was probably not mutagenic in vivo after acute exposure.
本研究旨在基于多终点遗传毒性测试系统确定2-甲基呋喃的遗传毒性。
将SPF级雄性SD大鼠（n = 30）随机分为六个处理组，即4个处理组（25、50、100和150 mg/kg）、一个对照组（植物油）和一个阳性组（N-乙基-N-亚硝基脲，80 mg/kg）。所有处理均通过灌胃连续给药3天。末次给药后3小时采集尾静脉血用于彗星试验。在第0天（灌胃前一天）、第14天和第28天进行Pig-a基因突变试验。在第0天和第4天使用流式细胞术在外周血中进行微核试验。
在彗星试验中，150 mg/kg剂量组外周血尾强度有统计学意义的增加。在Pig-a基因突变试验的2个时间点，各剂量组红细胞和网织红细胞的突变细胞频率无显著差异，在微核试验中各剂量组微核频率也无显著差异。
遗传毒性试验结果表明，急性暴露后2-甲基呋喃在体内可能无致突变性。
