El-Baz Farouk K, Salama Abeer A A, Hussein Rehab A
Plant Biochemistry Department, National Research Centre, 33 El Bohouth St., 12622 Dokki, Giza, Egypt.
Pharmacology Department, National Research Centre, 33 El Bohouth St., 12622 Dokki, Giza, Egypt.
Toxicol Rep. 2019 Nov 10;7:36-45. doi: 10.1016/j.toxrep.2019.10.017. eCollection 2020.
Several hepatic pathological conditions are correlated with the stimulation of hepatic stellate cells. This induces a cascade of events producing accretion of extracellular matrix components triggering fibrosis. , rich in carotenoids, was investigated for its potential antagonizing activity; functionally and structurally against thioacetamide (TAA) - induced hepatic fibrosis in rats. Adult male albino Wistar rats were treated with three dose levels of powder or extract (daily, p.o.); for 6 weeks, concomitantly with TAA injection. Serum levels of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), bilirubin and albumin were determined. Reduced glutathione (GSH), malondialdehyde (MDA), smooth muscle actin alpha (α-SMA) and collagen I hepatic contents were also estimated. Treatment with powder or extract caused a significant decline in serum levels of AST, ALT, ALP, bilirubin, MDA and hepatic contents of α-SMA and collagen I. Additionally, serum albumin and GSH hepatic content were highly elevated. Liver histopathological examination also indicated that reduced fibrosis, centrilobular necrosis, and inflammatory cell infiltration evoked by TAA. The results implied that exerts protective action against TAA-induced hepatic fibrosis in rats. The phytochemical investigation revealed high total carotenoid content prominently β-carotene (15.2 % of the algal extract) as well as unsaturated fatty acids as alpha-linolenic acid which accounts for the hepatoprotective activity.
几种肝脏病理状况与肝星状细胞的激活相关。这会引发一系列事件，导致细胞外基质成分积聚，进而引发纤维化。对富含类胡萝卜素的[具体物质未提及]进行了研究，以探究其在功能和结构上对大鼠硫代乙酰胺（TAA）诱导的肝纤维化的潜在拮抗活性。成年雄性白化Wistar大鼠接受三种剂量水平的[具体物质未提及]粉末或提取物（每日，口服）治疗；持续6周，同时注射TAA。测定了血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、胆红素和白蛋白的水平。还评估了还原型谷胱甘肽（GSH）、丙二醛（MDA）、平滑肌肌动蛋白α（α-SMA）和I型胶原在肝脏中的含量。用[具体物质未提及]粉末或提取物治疗后，血清中AST、ALT、ALP、胆红素、MDA以及肝脏中α-SMA和I型胶原的含量显著下降。此外，血清白蛋白和肝脏中GSH的含量大幅升高。肝脏组织病理学检查还表明，[具体物质未提及]减轻了TAA诱发的纤维化、小叶中心坏死和炎性细胞浸润。结果表明，[具体物质未提及]对大鼠TAA诱导的肝纤维化具有保护作用。植物化学研究显示，其总类胡萝卜素含量高，主要是β-胡萝卜素（占藻类提取物的15.2%）以及不饱和脂肪酸如α-亚麻酸，这解释了其肝脏保护活性。
