Plant Biochemistry Department, National Research Centre, Giza, Egypt.
Pharmacognosy Department, National Research Centre, Giza, Egypt.
Phytother Res. 2018 Feb;32(2):298-304. doi: 10.1002/ptr.5976. Epub 2017 Nov 29.
Microalgae represent a rich source that satisfies the growing need for novel ingredients of nutriceuticals, pharmaceuticals, and food supplements. Haematococcus pluvialis and Dunaliella salina microalgae are isolated from the Egyptian hydro-flora and are reported for their potent antioxidant activities. The cytotoxic activity of different fractions of both microalgae was investigated on 4 cell lines HePG2, MCF7, HCT116, and A549. The carotenoid rich fraction of H. pluvialis showed potent cytotoxic activity against colon cancer cell line and moderate activity against both liver and breast cancer cell lines. On the other hand, the carotenoid rich fraction of D. salina showed mild cytotoxic activity on breast and liver cancer cell lines. The carotenoid rich fraction of H. pluvialis was analysed using LC-DAD/ESI-MS and the major carotenoids were identified either free as well as bounded to fatty acids.
微藻是一种丰富的资源，可以满足人们对营养保健品、药品和食品补充剂的新型成分的日益增长的需求。雨生红球藻和盐生杜氏藻是从埃及水生植物中分离出来的，具有很强的抗氧化活性。研究了这两种微藻的不同馏分对 4 种细胞系 HepG2、MCF7、HCT116 和 A549 的细胞毒性作用。富含类胡萝卜素的雨生红球藻馏分对结肠癌细胞系表现出很强的细胞毒性活性，对肝癌和乳腺癌细胞系也有中等活性。另一方面，富含类胡萝卜素的盐生杜氏藻馏分对乳腺癌和肝癌细胞系表现出轻微的细胞毒性活性。使用 LC-DAD/ESI-MS 对富含类胡萝卜素的雨生红球藻馏分进行了分析，鉴定出的主要类胡萝卜素既可以游离存在，也可以与脂肪酸结合。
