系统性注射低剂量 LPS 可短暂改善地图状萎缩模型小鼠的视网膜功能和结构。
Systematic Injection of Low-Dose LPS Transiently Improves the Retina Function and Structure of a Mouse Model of Geographic Atrophy.
机构信息
Department of Ophthalmology, University of Florida College of Medicine, Gainesville, FL, USA.
出版信息
Adv Exp Med Biol. 2019;1185:57-62. doi: 10.1007/978-3-030-27378-1_10.
Geographic atrophy (GA), the advanced form of AMD, has been linked to oxidative stress within the RPE and with low-grade inflammation. The RPE-specific Sod2 knockout mouse model of GA develops increase oxidative stress and slow retinal degeneration. Mice of the SOD2::VMD2 genotype were injected subcutaneously with either saline or 3 mg/kg of lipopolysaccharide (LPS) at 8 weeks of age. Mice were evaluated by electroretinography (ERG) and spectral domain optical coherence tomography. Inflammatory cells within the retina were studied by CD45 immunofluorescence. Systemic low-dose LPS transiently, but significantly, improved both function and structure of RPE-specific Sod2 KO mice retina when compared to saline-injected mice. There was no difference in CD45 positive cells between saline and LPS treatment. Low-grade activation of the immune system leads to a preconditioning effect that transiently protects the retina of a mouse model of geographic atrophy.
地图样萎缩（GA）是 AMD 的晚期形式，与 RPE 内的氧化应激和低度炎症有关。GA 的 RPE 特异性 Sod2 敲除小鼠模型中，氧化应激增加，视网膜变性缓慢。SOD2::VMD2 基因型的小鼠在 8 周龄时皮下注射生理盐水或 3mg/kg 的脂多糖（LPS）。通过视网膜电图（ERG）和光谱域光学相干断层扫描评估小鼠。通过 CD45 免疫荧光研究视网膜内的炎性细胞。与生理盐水注射小鼠相比，系统给予低剂量 LPS 可短暂但显著改善 RPE 特异性 Sod2 KO 小鼠视网膜的功能和结构。CD45 阳性细胞在生理盐水和 LPS 处理之间无差异。免疫系统的低度激活导致预适应效应，可短暂保护地图样萎缩小鼠模型的视网膜。
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