关于人类细胞中DIS3L2对无义介导的mRNA衰变靶点的实验支持数据。

Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells.

作者信息

da Costa Paulo J, Menezes Juliane, Saramago Margarida, García-Moreno Juan F, Santos Hugo A, Gama-Carvalho Margarida, Arraiano Cecília M, Viegas Sandra C, Romão Luísa

机构信息

Department of Human Genetics, Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa, Portugal.

University of Lisbon, Faculty of Sciences, BioISI - Biosystems and Integrative Sciences Institute, Lisboa, Portugal.

出版信息

Data Brief. 2019 Dec 6;28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Feb.

DOI:10.1016/j.dib.2019.104943

PMID:31886366

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6921154/

Abstract

In this article, we present supportive data related to the research article "A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells" [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.

摘要

在本文中，我们展示了与研究论文《DIS3L2在人类细胞中对天然无义介导的mRNA衰变靶点的作用》[1]相关的支持性数据，该论文对本文所展示数据的解读可供查阅。实际上，我们在此分析了DIS3L2外切核糖核酸酶对无义介导的mRNA衰变（NMD）靶点的影响。具体而言，我们展示了以下数据：a）通过Northern印迹法和RT-qPCR监测的各种报告人类β-珠蛋白mRNA在HeLa细胞中DIS3L2水平改变前后的表达情况，以及b）通过重新分析来自UPF1缺失的HeLa细胞的公开可用数据并与DIS3L2缺失细胞中上调的转录本数据集进行进一步交叉参考而生成的失调转录本的基因表达水平。这些分析表明，DIS3L2调节了一部分NMD靶点的水平。这些数据对于对NMD机制感兴趣的研究人员可能具有重要价值。